Search results for "Upstream" in Articles / App Notes
Article
Host-Cell Protein Measurement and Control
In late phase (Phase III or commercial), cell line-specific platform assay or upstream process-specific ELISA assay is often required to mitigate the risk associated with a more generic commercial ass…
Article
Improving PAT for Biologics
Upstream and downstream
Although upstream processes tend to be more challenging and the sources of variability (raw materials and process) are reduced in downstream processes (e.g., after Protein …
Article
High-Throughput Process Development in an Historical Environment
About 100 delegates from 13 different countries joined the discussions on HTPD case studies in upstream, downstream, and formulations, as well as modeling, data analysis, and analytics. A new session …
Article
Application of Quality by Design to Viral Safety
Manufacturers of biopharmaceuticals are beginning to implement viral clearance steps in the upstream process for media and raw materials. These steps can include UV-C irradiation (6), gamma irradiatio…
Article
Concentrating Feed—an Applicable Approach to Improve Antibody Production
In this study, the authors evaluated different approaches to prepare highly concentrated feed media for fed-batch Chinese hamster ovary cell culture. By raising the pH or by adding surfactants, the fe…
Article
Trends in Downstream Bioprocessing
Improved upstream bioprocessing has created increasingly higher product titres and resulted in purification bottle necks. In addition, the recent increase in upstream single-use technologies has creat…
Article
Flexibility in Biopharmaceutical Manufacturing
Maintaining flexibility in biopharmaceutical manufacturing can deliver positive results.
By: Simon Chalk
BioPharm International
There is a high degree of consensus in the biomanuf…
Article
Free handbook for column packing and maintenance
Does large-scale column packing seem daunting? Impossible to get right? This new handbook can help! Packed with valuable guidance for using AxiChrom™, BPG and Chromaflow™ columns, you’ll disco…
Article
Innovations in Perfusion Bioprocessing
In this study, we present a method for deriving perfusion culture media by leveraging existing fed-batch media and feeds.
Furthermore, we illustrate the successful transfer of the develop…
Article
In silico optimization of AAV full/empty particle separation with AIEX
The separation of adeno-associated virus (AAV) with high yield and purity is an increasingly important challenge. Mechanistic modeling is the tool of choice to find the optimal AIEX chromatogr…