The separation of adeno-associated virus (AAV) with high yield and purity is an increasingly important challenge. Mechanistic modeling is the tool of choice to find the optimal AIEX chromatography method.
We present a guiding workflow showcasing in silico process optimization of full and empty AAV5 particle separation on strong AEX resins, such as Capto™ Q ImpRes or Capto™ Q with GoSilico™ chromatography modeling software to enable full separation performance.