Search results for "Upstream"
Article
High-Throughput Process Development in an Historical Environment
About 100 delegates from 13 different countries joined the discussions on HTPD case studies in upstream, downstream, and formulations, as well as modeling, data analysis, and analytics. A new session …
Article
Application of Quality by Design to Viral Safety
Manufacturers of biopharmaceuticals are beginning to implement viral clearance steps in the upstream process for media and raw materials. These steps can include UV-C irradiation (6), gamma irradiatio…
Article
Concentrating Feed—an Applicable Approach to Improve Antibody Production
In this study, the authors evaluated different approaches to prepare highly concentrated feed media for fed-batch Chinese hamster ovary cell culture. By raising the pH or by adding surfactants, the fe…
Article
Trends in Downstream Bioprocessing
Improved upstream bioprocessing has created increasingly higher product titres and resulted in purification bottle necks. In addition, the recent increase in upstream single-use technologies has creat…
Article
Flexibility in Biopharmaceutical Manufacturing
Maintaining flexibility in biopharmaceutical manufacturing can deliver positive results.
By: Simon Chalk
BioPharm International
There is a high degree of consensus in the biomanuf…
Article
Free handbook for column packing and maintenance
Does large-scale column packing seem daunting? Impossible to get right? This new handbook can help! Packed with valuable guidance for using AxiChrom™, BPG and Chromaflow™ columns, you’ll disco…
Article
Innovations in Perfusion Bioprocessing
In this study, we present a method for deriving perfusion culture media by leveraging existing fed-batch media and feeds.
Furthermore, we illustrate the successful transfer of the develop…
Article
In silico optimization of AAV full/empty particle separation with AIEX
The separation of adeno-associated virus (AAV) with high yield and purity is an increasingly important challenge. Mechanistic modeling is the tool of choice to find the optimal AIEX chromatogr…
Article
Extractables in Single-Use Systems Used in ADC Manufacturing, Part 2
We performed extractables studies on single-use chromatography and tangential flow filtration systems using common solvents in ADC manufacturing, DMA and DMSO. The low levels of extractables f…
Article
Exosome isolation by tangential flow filtration and size exclusion chromatography
We demonstrate a scalable workflow for the isolation of exosomes—a type of extracellular vesicle with significant potential for targeted drug/gene delivery. For research and development and di…