Search results for "Upstream"
Article
Downstream Processing for Cell-Based Therapies
Much of the research and development activity in the sector has addressed the upstream challenges with particular emphasis on large-scale cell expansion; however, equally important is the development …
Article
Reconciling Sensor Communication Gaps
For most of the history of the biomanufacture of therapeutic proteins, in-process measurement and control focused on upstream operations. Historically, cell culture and fermentation persist longest am…
Article
Generating a Fully Processed Antibody
About the Authors
Gregory Bleck is global head of R&D biologics; Dona York is director of upstream process development; and David Rabuka is global head of R&D chemical biology, all at Catalent.
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
The authors describe the ways in which manufacturers can mitigate the risks related to the integrity of recombinant transgenes expressed in CHO cells.
By Luhong He, Christopher Frye
Abstract
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Article
Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
The necessity to detach cells from a culture substrate during cell harvesting remains one of the most challenging steps in a cell-culture process.
By Marcos Simon, Juan J. Giner-Casares
Cell…
Article
Antibody Production in Microbial Hosts
The authors review the status of expression of antibodies in microbial hosts and present the recent advances in the production of aglycosylated antibodies in bacteria.
By Anurag S. Rathore, Jyot…
Article
Fluid Handling in Biopharma Facilities
For example, as the productivity of upstream processes has improved, the need to run a greater number of cycles through purification equipment has also increased, which increases buffer consumption. A…
Article
Video: Xcellerex XDUO 2500 Single-Use Mixer
Xcellerex XDUO 2500 is the first single-use large volume mixer from Cytiva to support up to 2500 L mixing. It is a robust, versatile, and easy to use single-use mixer that can be used for mixing ope…
Article
Host-Cell Protein Measurement and Control
In late phase (Phase III or commercial), cell line-specific platform assay or upstream process-specific ELISA assay is often required to mitigate the risk associated with a more generic commercial ass…
Article
Improving PAT for Biologics
Upstream and downstream
Although upstream processes tend to be more challenging and the sources of variability (raw materials and process) are reduced in downstream processes (e.g., after Protein …