Search results for " medium" in Articles / App Notes
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
Although Protein A resins introduced to the market in the last 10 years withstand low to medium concentrations of sodium hydroxide for cleaning, like 0.1 M sodium hydroxide and in some cases 0.5 M, th…
Article
Sources of Bioburden You Might Have Missed
What better place for a bacterial contamination to grow than in a well-aerated, nutrient-loaded cell culture medium? Downstream operations also have their share of risk. The open handling introduces m…
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Downstream Processing for Cell-Based Therapies
A biological safety cabinet usually is needed for the subsequent resuspension step, which involves discarding spent medium and resuspending in a suitable buffer. This process may be repeated multiple …
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Modeling Bioreactor Performance
In general, process-specific data must also be generated to perform these modeling studies because oxygen transfer and mixing times differ depending on the medium used and the bioreactor fill volume a…
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Mixing dynamics in a large volume single use mixer
Upstream applications such as medium preparation and harvest require fast and efficient mixing whereas downstream mixing operations used in purification and separation processes require sensitive mi…
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Selection Guide Size Exclusion Chromatography Columns and Media
Size exclusion chromatography (SEC), also called Gel filtration (GF), separates molecules on the basis of differences in size as they pass through a SEC medium packed in a column. A variety of media…
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A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
… improving therapeutic protein production systems with a focus on host-cell engineering,cell-culture medium, and process development.
While the aforementioned efforts successfully introduced clini…
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Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
…emporal resolution due to the formation of reactive oxygen species (ROS) that diffuse in the culture medium (13). Efforts in this direction have led to the development of a plasmonic substrate that p…
Article
Antibody Production in Microbial Hosts
The authors review the status of expression of antibodies in microbial hosts and present the recent advances in the production of aglycosylated antibodies in bacteria.
By Anurag S. Rathore, Jyot…
Article
Using Xcellerex™ mixing system as a slurry tank when packing chromatography columns
The capability of the mixing system to keep the chromatography medium (resin) in suspension and the possibility to integrate the system in column packing were studied. Further, an evaluation of the ef…