Search results for " medium" in Articles / App Notes
Article
Simple Fed-Batch Process Development for a Knockout Cell Line
CLICK HERE TO READ MORE >>
This workflow improved titers by 500% compared with Chinese hamster ovary cell culture in basal medium.
Article
Remote Monitoring and Big Data Advance Upstream Automation
Connected, integrated bioprocessing enterprises with greater data analytics capabilities are coming.
By Cynthia A. Challener
As upstream processing drives toward higher efficiencies in meeti…
Article
Mapping a Route for Cell and Gene Therapy Process Development
Moreover, the product design at the molecular level, the producer cell line, and the production medium can all be limiting factors for harvest titer. Most importantly, appropriate analytical methods a…
Article
Upstream PD Time-Saver: Optimize Early
Identify the right combination of culture medium, feed supplements, and feeding strategy early in upstream process development to conserve precious time and resources.
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Bulk response caused by the medium in which the analyte is dissolved is the major drawback but can be easily avoided with proper reference measurements (7, 8).
Analyte concentrations are measur…
Article
A Look at the Affinity Chromatography Landscape
A new affinity purification procedure using cell surface heparan sulfate proteoglycan and the non-ionic gradient medium iodixanol has also resulted in improved viral infectivity, efficient purificatio…
Article
Scalable Viral Vector Manufacturing
Also, the cell culture medium and any supplements need to be of clinical grade quality. From a regulatory perspective, it is recommended to avoid any raw materials that are of animal origin such as se…
Article
Understanding Validation and Technical Transfer, Part 2
…m various growing regions or should it be single-sourced? What level of roast should be used: light, medium, dark? How will the roasting be controlled? How will the beans be ground, and to what degre…
Article
Understanding Validation and Technical Transfer, Part 3
In any case, there is an incubation period where the cells grow in a medium that changes depending on the process. The incubation period is also variable depending on the process. Then the cells are c…
Article
Platform Technologies Improve Protein Expression
… for protein expression ranging from the choice of cell line, expression plasmid design, cultivation medium, growth conditions, equipment, scalability, stability, and matching purification capability…