Search results for " Protein A"
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Figure 1: Transgene copy number distribution profiles of cell lines expressing recombinant protein at generations of 0, 30, 45, and 60. Generation 0 (G0) represents the generation of a master cell ban…
Article
Best Practices for Selecting a Top-Quality Cell Line
Irina Shi - Stock.Adobe.com
Leveraging automation and a step-by-step approach are keys to success.
By Cynthia A. Challener
Cell lines determine the performanc…
Article
Fermentation for the Future
Improving efficiency, value chain, quality, and protein complexity with advanced bioprocess development.
Jan 01, 2015
By Cynthia Challener, PhD
Microorganisms—bacteria and yeast—have b…
Article
Evolution of the Monoclonal Antibody Purification Platform
Typical steps include:
• Protein A chromatography for capture of the product and removal of host cell-related impurities (host cell proteins and DNA). Though extremely efficient and effective,…
Article
Quality by design for biotechnology products—part 1
…sidual medium supplements such as protein hydrolysates, and components such as nucleases or residual Protein A from chromatography resins used in the purification process.
There are multiple appr…
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Recently, automated 2D Protein A–SEC methods for the purification and analysis of MAbs have been developed.28,29 In these methods, multiple protein separation steps were performed automatically. Sampl…
Article
A Look at the Affinity Chromatography Landscape
…erved as a method for removal of process-related impurities such as host-cell proteins and DNA, with Protein A the most widely used resin. Resin suppliers have in recent years been challenged to incr…
Article
Improving Process-Scale Chromatography
The development of new resin chemistries including enhancements to Protein A for monoclonal antibody (mAb) capture, increasing use of automation, implementation of single-use technologies, and explora…
Article
Process Chromatography: Continuous Optimization
…obal product marketing manager for Cytiva, are typically purified using a platform approach in which protein A chromatography resins are used in the first capture step. Polishing is then achieved usi…
Article
Antibody Production in Microbial Hosts
coli, the Sec-dependent pathway has been used for protein secretion because of its abundance, although accumulation of protein aggregates may occur due to the generation of premature proteins before s…