Search results for " Protein A"
Article
Cell-free Expression Systems Pose Cell Culture Alternative
Cell-free expression is promising in preclinical applications, but still presents challenges to scale up for commercial production.
Feliza Mirasol
Among the challenges in upstrea…
Article
Advances in Engineering of Protein-Based APIs
Similarly, miniaturization and high-throughput versions of protein assays will reduce analysis times and enable exploration of wider design spaces. Stevens points to nanofluidic characterization assay…
Article
Concentrating Feed—an Applicable Approach to Improve Antibody Production
Antibody titer was measured using high-performance liquid chromatography (HPLC) (Waters Corporation) equipped with a protein A column (Applied Biosystems). The protein charge profile was analyzed by i…
Article
Innovative Chromatography Resins Can Improve Purity and Quality
A generic capture step for mAbs and Fc fusion proteins has been robustly established by classical Protein A affinity resins, says Oliver Schub, director of Business Development at Navigo Proteins, a s…
Article
Continuous Manufacturing: A Changing Processing Paradigm
To standardize downstream operations, he says one option would be “the rational design of highly specific ligands for the capture step analogous to Protein A in mAb purification” (3).
Costs of ado…
Article
Reimagining Affordable Biosimilars
With new configurations and solid-state designs, SPR has become a workhorse in protein and nucleic acid applications. The sensor surfaces have been modified to allow cell-based binding to mimic real-t…
Article
Process Chromatography Selection for Downstream Processing Applications
In the case of Fc-fusion proteins, Protein A can still be utilized as a capture step.
Levison (Pall): [Some commonly used separation technologies include] ion-exchange, mixed-mode, hydrophobic int…
Article
Novel Vaccine Technologies Meet the Need for Pandemic and Therapeutic Solutions
Extensive DNA and protein analysis is required to help avoid this problem, and further testing is conducted to ensure that no pathogens from the eggs are transferred to the vaccine. All of this testin…
Article
Emerging Therapies Test Existing Bioanalytical Methods
Drug developers must understand the complex bioanalytical assays for cell- and gene-therapy drug development programs and ensure that partners have the specialized expertise needed for complex thera…
Article
Leveraging Computational Models of Glycosylation for Biopharma QA
Close collaboration between academic and industrial groups is vital to ensuring glycosylation models are fit for deployment.
By Ioscani Jiménez del Val
Good Studio/S…