Search results for " yield"
Article
A Platform Approach for the Purification of Domain Antibodies (Dabs)
This Application note describes a three-step purification process of a domain antibody (Dab) expressed in the periplasm of E. coli. First, a capture step using Capto™ L was used to reduce E. coli host…
Article
Considerations in depth filter scale-up
Analysis includes pressure profiles, turbidity and particle reduction, yields, and impurity removal. This data will help process development scientists and engineers understand the structure of these …
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In silico optimization of AAV full/empty particle separation with AIEX
The separation of adeno-associated virus (AAV) with high yield and purity is an increasingly important challenge. Mechanistic modeling is the tool of choice to find the optimal AIEX chromatogr…
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Purification strategies for antibody-derived entities: bispecifics and fragments
Purification platforms used for many monoclonal antibodies (mAbs) need to be adopted for antibody variants to simplify protocols and improve purity and yield. The safety and efficiency requirement of …
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Alternative solutions to separate AAV full and empty capsids using anion exchange
Key for a successful process are high overall yields of full capsids with effective empty capsid reduction and efficient impurity removal.
Here we present high-performance anion exchange (AEX) chr…
Article
A Closer Look at Affinity Ligands
The matrix is typically in the form of small 50–150 micron beads that are highly porous to provide a large surface area, maximizing antibody binding potential for higher yields from fixed-volume colum…
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Discovering the unknown: How does in silico process development change PD scientists’ work?
This resulted in improvements in purity and yield of a mixed mode chromatography purification step, and doubled productivity. Mechanistic modeling can also support early-phase development. The technol…
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High-concentration monoclonal antibody drugs: manufacturing challenges
Solutions that maintain quality, maximize process yield and product recovery are available and can be introduced to adapt an existing manufacturing platform to the new process requirements.
Downloa…
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Future-proof your AAV process with a complete producer cell line – just induce and grow
In this webinar:
• Discover the drawbacks of using a cell line that’s not complete
• See how to use the ELEVECTA™ platform to produce high-quality AAV at high yield for indications with large p…
Article
How to develop a scalable rAAV process
The key for a successful process are high overall yields of full capsids with empty capsid reduction and efficient impurity removal.
In this webinar you will learn:
• Common pitfalls for rAA…