Search results for " capture"
Article
The Development and Application of a Monoclonal Antibody Purification Platform
Protein A chromatography was used in the initial capture step, where the antibody in cell culture broth was loaded at neutral pH, washed with various solutions, and eluted at a lower pH value. The elu…
Article
Optimizing KiH bispecific antibody chromatography steps
This web article describes how Bioprocessing Technology Institute (BTI), Singapore together with scientists at Cytiva developed an optimized capture step for two closely related knob-into-hole (KiH) b…
Article
Process development using a nonaffinity method vs Cytiva™ Protein Select™ resin
The first method is using nonaffinity chromatography steps (AIEX + heat treatment + HIC), while the second method is using the novel affinity Cytiva™ Protein Select™ resin for the capture step, follow…
Webcast
Webinar: Intensifying Downstream Bioprocessing With Continuous Technologies
We will discuss:
-Intensification of capture chromatography and the benefits of coupling with viral inactivation
-How a single flow through chromatography step can simplify polishing
-The rol…
Article
Advances in Sterilization Technologies for Overcoming Viral-Vector Manufacturing Challenges
“For a 50-L bioreactor run, the intermediate downstream processing volumes can already be below 2 L after the capture steps, but single-use mixers currently on the market start at 50 L,” she explains.…
Article
Webinar: Intensifying downstream bioprocessing with continuous technologies
We will discuss:
• Intensification of capture chromatography and the benefits of coupling with viral inactivation
• How a single flow through chromatography step can simplify polishing
• The …
Article
Life cycle assessment of the protein A process in mAb manufacturing
Here we present a cradle-to-grave LCA for protein A chromatography resins in the capture step of mAb manufacturing. The focus is on the resin use phase, although resin manufacturing, packaging, distri…
Article
Discovering the unknown: How does in silico process development change PD scientists’ work?
So experiments that are performed for modeling aim to capture the dynamics of the interaction between the biomolecules and the ligand. This can be realized by running, for example, linear gradient exp…
Article
Get Started: Purification of mAb Variants - eLearning
- Understand which resins are suitable for the first capture step.
- Discover the different strategies for removal of product- and process-related impurities in the polishing steps.
ACCESS TRAIN…
Article
Chromatography innovations for the diversified antibody pipeline
MabSelect™ VH3 resins provides efficiency in capture of bispecific antibodies and antibody fragments such as Fab, scFv, and VHH. Discover how we’re supporting the diverse antibody pipelines with chrom…