Search results for " DNA"
Article
Moving PAT from Concept to Reality
Numerous at-line analyses evaluate host cell proteins (HCPs), DNA, viable cell density, bioburden, aggregates, product concentration, and particulates.
One of the greatest difficulties with many c…
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Process Chromatography: Continuous Optimization
Host-cell proteins, host-cell DNA, other process-related contaminants, and product-related impurities (e.g., aggregates, incorrectly modified or unfolded proteins, etc.) must be removed. A combination…
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Ensuring the Biological Integrity of Raw Materials
…y of California San Francisco, tested eight viral vaccines using polymerase chain reaction (PCR) and DNA sequencing, and found that three of the vaccines contained unexpected viral sequences. (4) One…
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Changing the Culture of Cell Culture: Applying Best Practices and Authentication to Ensure Scientific Reproducibility
Another DNA profiling test of cell-line identity uses single nucleotide polymorphism (SNP) variations between members of the same species within a specific locus, which are conserved during evolution …
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Fermentation for the Future
It is also useful for the production of cytokines, growth factors, and plasmid DNA. Reinisch also notes that media costs for microbial fermentation are lower, although media costs for cell culture hav…
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Setting Standards for Biotech Therapeutics in India
Approval of the first recombinant DNA product, human insulin, occurred only in 1982. The experience is even more limited in India. The government, through agencies such as the Department of Biotechnol…
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Comparing Protein A Resins for Monoclonal Antibody Purification
Table two
The results show that the step yield was consistently over 95%, and high log-reduction factors of host-cell proteins (HCP) and DNA were achieved (see Figure 1). In this study, car…
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Advances in Sterilization Technologies for Overcoming Viral-Vector Manufacturing Challenges
Sterility must be assured for all biologic drug products. Terminal sterilization achieved via treatment with heat, radiation, or certain chemicals (vaporous hydrogen peroxide, vaporous peracet…
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A Closer Look at Affinity Ligands
Today, Protein A ligand is manufactured and purified on a large scale using recombinant DNA technology. Purfied Protein A ligand is covalently coupled (conjugated) to a solid, porous support to form a…
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Get Started: Purification of mAb Variants - eLearning
These molecules show wider variation in binding affinities and are often more challenging to separate from impurities, such as host cell proteins (HCP), host DNA, aggregates, and isoforms.
In this …