Search results for " recombinant" in Articles / App Notes
Article
Generating a Fully Processed Antibody
Protein conversion by FGE
Figure 1: Formylglycine-generating enzyme (FGE) protein recognition site and conversion process in the context of a recombinant protein of interest.
Formylglycine-gene…
Article
Affinity Chromatography Handbook: Vol. 1 Antibodies
The use of recombinant technology opens up the potential to create an infinite number of combinations between immunoglobulins, immunoglobulin fragments, tags and selected proteins, further manipulatin…
Article
A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
…omponents of cell-culture media used in a Chinese hamster ovary (CHO) cell manufacturing process for recombinant proteins (11). Although, both NIR and Raman spectroscopy are fast and non-destructive,…
Article
Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
Will the facility be manufacturing monoclonal antibodies, recombinant proteins, vaccines, antibody-drug conjugates, or fragment antibodies? Also, will the products be mammalian cell-derived or microbi…
Article
Tools for Continuous Bioprocessing Development
Cells were genetically modified to produce different recombinant proteins in response to different chemical inducers and it was shown that by testing the synthetic circuits in turbidostatic steady sta…
Article
Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
Data were available for 15 measurements over 16 time points for 20 cultures (19 Chinese hamster ovary cell lines) making the same recombinant monoclonal antibody (mAb) giving a 240-dimensional data se…
Article
GMP Challenges for Advanced Therapy Medicinal Products
“Manipulation of cells and use of recombinant nucleic acids may bear unknown risks, which may not be solvable through standardization or quality control,” Salmikangas added.
A major problem area wi…
Article
Implications of Cell Culture Conditions on Protein Glycosylation
The choice of host-cell line for recombinant protein production depends first on the protein’s molecular properties. Certain bacteria can be used for production of the simplest proteins, those that ar…
Article
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
Advanced antibody engineering has resulted in the ability to manufacture new recombinant bispecific antibodies suitable for therapeutic use. FDA has approved a bispecific antibody—Amgen’s Blincyto (bl…
Article
Antibody Fragments and Their Purification by Protein L Affinity Chromatography
In the case of recombinant full-length antibodies with suitable Fc regions, two or three column purification processes centered around Protein A affinity chromatography have proven to be fast, efficie…