Search results for " recombinant" in Articles / App Notes
Article
Development of Purification for Challenging Fc-Fusion Proteins
Materials and Methods
Purification techniques
Feed stock: Fc-fusion proteins with an isoelectric point (pI) ranging from four to seven were expressed in recombinant Chinese h…
Article
Evaluation of HEK293 Cell Growth and Adenovirus Productivity in HyClone™ CDM4HEK293 Medium
Human embryonic kidney 293 (HEK293) cells are widely used in production of recombinant viruses and proteins because of their stable growth, high transfection level, and ability to produce high viral t…
Article
Process Chromatography: Continuous Optimization
… purification process making it simpler and more economical for a variety of molecules from mAbs, to recombinant proteins, to viruses.
Hydrophobic interaction resins separate molecules based on d…
Article
Managing Residual Impurities During Downstream Processing
Some specifics on residual HCPs
The presence of HCPs in a biologic material is inevitable given that the main route of production for biopharmaceuticals is through recombinant technology via a host…
Article
Fusion Proteins Pose Manufacturability Challenges
Making proteins ‘druggable’
Many proteins have short half-lives in human serum, making them unsuitable as recombinant protein drugs. When constructed as fusion proteins, however, they may become ‘…
Article
Single-use Bioreactors Have Reached the Big Time
“[Cytiva] is taking its experience in single-use bioreactor technologies for monoclonal antibody, recombinant protein, and vaccine processes and applying it in our Xuri line of cell therapy equipment …
Article
Biopharma in 2016: Higher Quality Drugs, Less Expensive Manufacturing
…ologies in Downstream Process Intensification
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
A Second Modular Bi…
Article
Improving Process-Scale Chromatography
She points to several new types of affinity chromatography resins introduced to the market in recent years that target recombinant plasma proteins, gene therapy applications, and different types of an…
Article
Process Chromatography Selection for Downstream Processing Applications
…
Björkman (Cytiva): If one excludes mAbs, then the molecules of interest are generally peptides, recombinant proteins, or vaccines. For these molecules, chromatographic techniques such as ion-exch…
Article
Perfusion in the 21st Century
By the early 2000s, perfusion was largely used to manufacture only products such as recombinant blood-clotting factors and enzyme treatments for rare metabolic storage disorders such as Fabry’s diseas…