Search results for " purification"
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Host-Cell Protein Measurement and Control
FDA expects “Whenever possible, contaminants introduced by the recovery and purification process should be below detectable levels using a highly sensitive analytical method” (7). The European Medicin…
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Modular Manufacturing Platforms for Biologics
Caliber Biotherapeutics employs six pods for every step of its downstream purification process,” he adds. Backstrom says that the flexibility of the OSD line allows site managers to seamlessly add pod…
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High-Throughput Process Development in an Historical Environment
A new session this year was the HTPD Olympiad, for which five teams had signed up to develop a purification process for a domain antibody (Dab). The challenge was to use HTPD workflows for maximized D…
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Recently, automated 2D Protein A–SEC methods for the purification and analysis of MAbs have been developed.28,29 In these methods, multiple protein separation steps were performed automatically. Sampl…
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Quality by design for biotechnology products—part 1
…sates, and components such as nucleases or residual Protein A from chromatography resins used in the purification process.
There are multiple approaches to assessing the criticality of quality at…
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Selected courses from cell culture and downstream process development to scale-up and manufacturing
…scaling
Filtration in bioprocessing
Purification of mAb variants
DoE in protein purification
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Recap: Chromatography + filtration virtual summit 2024
Don’t worry – here are the highlights and session recordings from the event on optimizing purification and filtration for mAbs, antibody variants, recombinant proteins, and gene therapy.
See highli…
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Alternative solutions to separate AAV full and empty capsids using anion exchange
Adeno-associated virus (AAV) is the main vector for gene therapy and there is need for scalable, cost-efficient, and robust chromatography-based purification. Key for a successful process are …
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Video: The solution to smarter column packing
Packing modern chromatography resins at large scale requires a column capable of axial compression to ensure stable, reproducible packed beds. AxiChrom™ columns are low-pressure, axial compressi…
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FAQs about Cytiva™ Protein Select™ technology
One chromatography step does the affinity purification, a traceless tag cleavage, and the tag removal. The pure protein obtained is in its native state with no traces of the tag amino acids. The proto…