Search results for " purification"
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Exploring the world of antibodies: mAbs, Fabs, dAbs, and bsAbs
Developing purification processes for antibody variants is not always straightforward and the platform approaches conveniently used for many traditional mAbs cannot be used.
Learn more about develo…
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Dear lentivirus: We have not forgotten about you and how to improve your polishing recovery
Vector purification is very challenging due to low stability of these enveloped viruses, which are sensitive to low pH, high salt, temperature, shear forces, and other factors.
To maximize physica…
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How to develop a scalable rAAV process
Adeno-associated virus (AAV) is the most common vector for gene therapy, yet there’s much room to improve production, purification, and analytics. Here we present the latest updates to our wor…
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How to program, run and evaluate a design of experiments (DoE)
An application example showcases how to optimize an antibody purification for low dimer-and-aggregate and host cell proteins (HCPs) content while achieving best possible yields.
Take the course now…
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Choose your Optimal Wavelength for Chromatography
… security of ӒKTA avant™ through to the bioprocess-scale systems – the ability to scale up your purification is readily available
For customers with a focus on emerging molecules, proteins, pe…
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Dynamic Binding Capacity in Fiber-Based Chromatography
Fiber chromatography matrices such as Fibro PrismA can bind a large amount of protein in a fraction of the purification time required for an affinity resin. Just like with resins, the dynamic binding …
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From cells to purified capsids: How to develop a scalable rAAV process
Adeno-associated virus (AAV) is the most common vector for gene therapy, yet there’s much room to improve production, purification, and analytics. In this webinar we present the latest upd…
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Sign-up to join Chromatography Virtual Summit 2022
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Join presentations and discussions with fellow process developers on recent advances in antibody purification and mechanistic modeling.
The summit will include presentations, each with l…
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New hydrophobic interaction chromatography (HIC) handbook
The role of this technique in protein purification in the lab and at bioprocess scale, the principle of the technique, and the equipment and resins needed are described in this detailed handbook.
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Optimization of an antibody polishing step
But what if DoE fails to find robust process conditions? This article shows how to achieve higher yields using mechanistic models to optimize an antibody purification process.
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