Search results for " tag" in Articles / App Notes
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Bind, Cut, Elute — Easy Affinity Tag and Protease Removal
The tag is cleaved while the protein is still bound to the affinity resin.
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Purifying Tagged Proteins: Learn the Basics
When and why should you use it? Learn the basics in our quick guide to tagged protein purification.
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Affinity chromatography Handbook: Vol. 2 Tagged Proteins
The advantages of using a protein/peptide tag fused to the recombinant protein to facilitate its purification and detection are now widely recognized. In some cases, tags may improve the stability and…
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Generating a Fully Processed Antibody
…GE cell line to produce cell lines and processes capable of expressing and converting high titers of tagged antibody. The FGE cell line needed to have all the normal phenotypic traits of the base CHO…
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Optimization of Protein Expression in Escherichia Coli
Affinity tagging
The addition of affinity tags may aid the detection and purification of target proteins. Common affinity tags include poly-His, FLAG, c-Myc, poly-Arg, and StrepII tags. The tag cou…
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FAQs about Cytiva™ Protein Select™ technology
Purifying a protein tagged with this technology is simple. One chromatography step does the affinity purification, a traceless tag cleavage, and the tag removal. The pure protein obtained is in its na…
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Top challenges in recombinant protein purification process development
One example of this is a technology that I've recently presented at PepTalk and PEGS, that is actually a tag affinity solution that will allow for customers to use this product but not have any amino …
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A Closer Look at Affinity Ligands
Elliott (BioCina): A very interesting recent development from Cytiva is the ProteinSelect system which consists of a tag protein sequence added to the N-terminus of a target protein and an affinity li…
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Webinar about a new affinity chromatography technology for recombinant proteins
During a simple affinity step, the protein self-cleaves from the tag and elutes with no residual tag amino acids. The protocol does not require the use of a protease, and the same buffer can be used f…
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Video: Standardize recombinant protein purification process development
During an affinity step performed with Cytiva Protein Select resin, the protein self-cleaves from the tag and elutes with no residual tag amino acids.
This technology standardizes purification duri…