Search results for " upstream" in Articles / App Notes
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Automating the Biomanufacturing Process
Automation implementation
For more than 15 years, biopharmaceutical manufacturers have been implementing automation and control systems, both upstream and downstream, says Christoph Lebl, head of …
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Modeling the Degradation of mAb Therapeutics
They result in instabilities that can occur at various stages of product development, including upstream processing (during protein expression in cell culture), downstream processing (purification), p…
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Challenges in Cell Harvesting Prompt Enhancements
As with centrifugation, filtrations processes, as well as filter manufacturers, have been challenged by the increasing titers and cell densities ubiquitous now in upstream cell-culture processing. The…
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Making the Move to Continuous Chromatography
Continuous chromatography systems are designed to be loaded continuously, which is a useful approach when the purification step is connected to an upstream perfusion bioreactor. There are several diff…
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Full Single-Use Downstream Process? Not Any Time Soon.
But where we see full single-use upstream processes, downstream is often a hybrid solution. Here’s why SUTs will not completely replace reuse systems in the near future.
Continue reading in this…
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Reducing Cross-Contamination Risks in Process Chromatography
Reducing Cross-Contamination Risks in Process Chromatography
Single-use systems provide replaceable fluid paths.
By Cynthia A. Challener
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Process Chromatography: Continuous Optimization
“An increase in binding capacity will be beneficial for process economy reasons when purifying steadily increasing upstream titers,” he states. Current protein A resins are also exposed to feeds with …
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Managing Residual Impurities During Downstream Processing
“It is essential, therefore, to develop online upstream and downstream monitoring systems for key process and product impurities, including product degradents, aggregates etc., such that if necessary,…
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Fusion Proteins Pose Manufacturability Challenges
Combining two different proteins in one molecule can lead to instability, which creates manufacturing challenges during both upstream and downstream processing. Solutions for overcoming these issues a…
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Sources of Bioburden You Might Have Missed
The risk is most acute in the upstream process, which should not be a surprise. What better place for a bacterial contamination to grow than in a well-aerated, nutrient-loaded cell culture medium? Dow…