Search results for " resin"
Article
Development of Purification for Challenging Fc-Fusion Proteins
In this study, both CEX and HIC resins were evaluated in a flow-through mode as a polishing step in purification of Fc-fusion proteins that previously proved challenging to purify using conventional A…
Article
Process Chromatography Selection for Downstream Processing Applications
Important factors such as column design, resin development, and automation have all played a role in simplifying the purification process, in some cases reducing it from five steps to three (1). Selec…
Article
SEC in the Modern Downstream Purification Process
Kevin O'Donnell
BioPharm International
Volume 3, Issue 28
Since the introduction of commercial chromatography resins approximately six decades ago, size-exclusion chromatography (SEC) ha…
Article
Evolution of the Monoclonal Antibody Purification Platform
Though extremely efficient and effective, the step primarily suffers from the significantly high cost of Protein A resins in comparison to other popular modes of chromatography (such as ion exchange).…
Article
Top Tips for Successful Development of Antibody Chromatography Processes
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Jeanne Linke Northrop: Hello, I…
Article
Technology Innovations Improve Process Chromatography Performance
Deorkar also notes that there is a lack of diversity in the supply chain of Protein A resin, which is used in the first downstream chromatography step in monoclonal antibody (mAb) manufacturing. “That…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
The HTS method has also been applied to the development of other purification processes for unique biotherapeutic modalities where screening of a large number of resins may be required.
Another cons…
Article
Improvements in Protein A Chromatography
However since 1978, the capacity and productivity of Protein A resins have improved at a rate of about 5% a year. The net effect of this improvement is that newer resins are an order of magnitude bett…
Article
Addressing the Complex Nature of Downstream Processing with QbD
“For instance, protein A or affinity resins in general, as well as flow through steps, display these properties since these steps in most cases build on a fundamental understanding of the physiochemic…
Article
The Challenge of Disruptive Technologies in Bioprocessing
“The Thermo Scientific POROS CaptureSelect technology provides highly selective affinity resins with antibody-like specificity, which can significantly improve the biomolecule purification process, ma…