Search results for "chromatography" in Articles / App Notes
Article
Optimization of Protein Expression in Escherichia Coli
For example, MBP will bind to amylose-agarose, while GST will bind to glutathione-agarose for purification by affinity chromatography. Similar to affinity tags, these fusion partners must be cleaved o…
Article
USP Publishes Monoclonal Antibody Guidelines
The material is an IgG1, 2mg/vial lyophilized presentation, which is used in the size-exclusion chromatography assay (SEC) and the capillary sodium dodecyl sulfate (SDS) electrophoresis (reduced and n…
Article
Implementation of Raw Material Control Strategies in the Manufacture of Single-Use Bioprocessing Containers
Quantification of TBPP, oxidized TBPP, and bDtBPP was performed with ultrahigh-performance liquid chromatography (UHPLC) with Exactive Orbitrap high-resolution accurate mass mass spectrometry (HRAM MS…
Article
DoE Provides Benefits, but Preparation Is Necessary
It is also important to reduce the data to single-value results as often as possible, such as the percent monomer or aggregate as determined using size exclusion chromatography-high performance liquid…
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A Q&A With Phil Lester, co-organizer of the HTPD Conference
For the Olympiad, Cytiva created a kit containing a target protein in a crude feedstock along with a variety of chromatography media and suggested analytical methods. The goal is to purify the target …
Article
Defining Critical Quality Attributes for Monoclonal Antibody Therapeutic Products
Figure 1: The total ion current (TIC) chromatogram obtained from on-line reverse phase-high-performance liquid chromatography with electrospray mass spectrometry (RP-HPLC/ES-MS) analysis of a monoclon…
Article
Challenges in Analytical Method Development and Validation
Experts give insight on method transfer, QbD, and regulations for analytical method development and validation for biopharmaceuticals.
The manufacture of biopharmaceuticals presents …
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Q&A with Industry Leader Konstantin Konstantinov, Genzyme Vice-President
But now several companies, including Cytiva, are making unit operations for continuous chromatography based on switching columns. So we’re beginning to see a movement toward integrated up and downstre…
Article
Outsourcing Trends in Biopharmaceutical Manufacturing
From expertly packing chromatography columns, to providing higher-quality liquid media and buffers at large-scale, suppliers with technical competence are taking on some of the industry’s most challen…
Article
Best Practices in Adopting Single-Use Systems
Key considerations for implementing single-use components or platforms when moving from research to process development.
The adoption of single-use systems in biopharmaceutical production or…