Search results for "chromatography" in Articles / App Notes
Article
Using Quality by Design to Develop Robust Chromatographic Methods
In the case examples discussed in this article, following such a discussion between R&D and receiving laboratory analytical scientists, reversed-phase high-performance liquid chromatography (RP-HPLC) …
Article
Biopharma Moves to Integrated, Single-Use, Downstream Processing
Chromatography is the last purification unit operation to move to disposability and has seen only limited use, according to Gebski. She notes that the adoption of pre-packed, limited-use chromatograph…
Article
Process development using a nonaffinity method vs Cytiva™ Protein Select™ resin
The first method is using nonaffinity chromatography steps (AIEX + heat treatment + HIC), while the second method is using the novel affinity Cytiva™ Protein Select™ resin for the capture step, follow…
Article
Advances in Sterilization Technologies for Overcoming Viral-Vector Manufacturing Challenges
Chromatography systems have presented challenges as well, as most were developed for monoclonal antibody processes with starting volumes of 1000 L. Suppliers are, Langhauser observes, working to addre…
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Purification strategies for antibody-derived entities: bispecifics and fragments
The diversity of antibody variants in today’s pipeline presents unique challenges for purification. Purification platforms used for many monoclonal antibodies (mAbs) need to be adopted for ant…
Article
Life cycle assessment of the protein A process in mAb manufacturing
Here we present a cradle-to-grave LCA for protein A chromatography resins in the capture step of mAb manufacturing. The focus is on the resin use phase, although resin manufacturing, packaging, distri…
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Alternative solutions to separate AAV full and empty capsids using anion exchange
Here we present high-performance anion exchange (AEX) chromatography separation for several AAV serotypes using step-elution mode. Optimized protocols and conditions for both resin and membrane format…
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Instructional Videos for Bioprocess Equipment
Cytiva offers free on-demand instructional videos that will help you with operating bioreactors, chromatography and filtration systems, including flow kit installation on single-use systems. Modules a…
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Video: The solution to smarter column packing
AxiChrom™ columns are low-pressure, axial compression chromatography columns for use during process development and manufacturing of biopharmaceutical products. The columns provide automated packing a…
Article
Get Started: Purification of mAb Variants - eLearning
You’ll also find useful tips for different chromatography steps.
Key learnings:
- Learn the important considerations for selecting a purification protocol.
- Understand which resins are suita…