Search results for "chromatography" in Articles / App Notes
Article
Will My Buffers Work When Using an Inline Conditioning System?
The system can be used off-line as a standalone central buffer preparation station to feed different processes in the facility or in-line connected to a chromatography column using a built-in chromato…
Article
Fusion Proteins Pose Manufacturability Challenges
“These fusion proteins include an Fc region, are taken from an IgG, that extends the serum half-life of the fusion partner, facilitates expression, and allows purification by protein A affinity chroma…
Article
Accelerated flavivirus vaccine production with modern tools and solutions
Single-use production bioreactors and chromatography purification columnsmitigate cross-contamination risk and support increased operator safety, while reducing time to market by eliminating costly an…
Article
Evaluating E&L Studies for Single-Use Systems
Analytical methods
In the three case studies, the following analytics were applied:
• Headspace sampling/gas chromatography/mass spectrometry (HS/GC/MS) and gas chromatography/mass…
Article
A Platform Approach to Purification of Antibody Fragments
However, with recent developments of novel affinity chromatography resins, there are new emerging possibilities. Here, we present affinity chromatography resins that offer the possibility of a platfor…
Article
Single-use technologies in downstream process intensification
When using conventional chromatography solutions, cleaning and cleaning validation consumes a considerable amount of time and facility resources. Cleaning-in-place protocols are performed before, duri…
Article
Automated harvesting and 2-step purification of unclarified mammalian cell-culture broths containing antibodies
We report on a new integrated platform for automated harvesting of whole unclarified cell-culture broths, followed by in-line tandem affinity-capture, pH neutralization and size-exclusion chromatograp…
Article
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
MS is further used in combination with high-performance liquid chromatography (HPLC) and other chromatographic methods to detect and localize amino acid modifications (13). Chromatography techniques a…
Article
Use of Multivariate Data Analysis in Bioprocessing
…spectroscopic measurements, analysis of data profiles from unit operations such as cell culture and chromatography, quantitative assessment of process comparability, root cause analysis, and raw mate…
Article
Viral Clearance Challenges in Bioprocessing
…oval could be demonstrated under standard conditions like low pH inactivation, anion exchange (AEX) chromatography, or virus-retentive filtration? Further studies need to be performed to answer these…