Search results for "chromatography" in Articles / App Notes
Article
Technical and Process Economical Aspects of Using Capto™ Q and ReadyToProcess™Adsorber Q in mAb Polishing
Compared with conventional chromatography, using stainless steel columns, the use of ReadyToProcess Adsorber membranes can help reduce capital investment, consumption of buffers and other process liqu…
Article
Development of column packing methods based on pressure flow measurements
A typical problem to be addressed during scaleup is the loss of wall support for the chromatography resin as a result of the large change in aspect ratio, causing larger compression of the resin and a…
Article
Analysis of Glycosylation in Biosimilars
Typically, this involves breaking the sample down into its individual monosaccharides, which can be identified and quantified by the retention time and fragmentation pattern using gas chromatography-m…
Article
Optimize Column Loading Strategy to Gain Productivity in Protein Purification
Have you ever applied the concept of variable loading to gain productivity? By varying residence time (RT) over the loading phase, productivity from an affinity chromatography step can be significantl…
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A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
The micellar electrokinetic capillary chromatography (MEKC) was used an alternative approach to spectroscopic methods for media identification. MEKC was successfully validated and employed for the ide…
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Implications of Cell Culture Conditions on Protein Glycosylation
For example, a high pH anion-exchange chromatography procedure with pulsed amperometric detection (HPAEC–PAD) can be used for the analysis of N-glycans. An example of this can be seen in the N-glycans…
Article
Technologies and Practices Must Evolve to Meet Demand
Chromatography systems, filtration, and centrifuges received marks of “excellent” from more than 20% of the respondents (Table I). Sampling systems, single-use systems, and sterile sampling received “…
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Comparing Protein A Resins for Monoclonal Antibody Purification
As a consequence, downstream processing must accept and handle higher titers of mAbs in harvested cell-culture fluid (HCCF), and vendors of mAb purification technologies must develop chromatography re…
Article
Bioprocessing Advances in Vaccine Manufacture
This trend includes 54% considering high-capacity chromatography resins; 44%, single-use filters; 38%, automated buffer dilution systems; and 35%, single-use tangential flow filtration.Other advances …
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In silico optimization of AAV full/empty particle separation with AIEX
Mechanistic modeling is the tool of choice to find the optimal AIEX chromatography method.
We present a guiding workflow showcasing in silico process optimization of full and empty AAV5 particle se…