Search results for "chromatography"
Article
N-Glycan Analysis of Biotherapeutic Proteins
The intact mass of the different glycoforms of a glycoprotein can be characterized by high-resolution accurate mass-mass spectrometry (HRAM-MS), coupled to reverse-phase liquid chromatography (RP-LC).…
Article
Downstream Processing Continues to Worry CMOs
(Figure courtesy of author)
Chromatography and ultrafiltration
When it comes to specific steps that contribute to downstream-related capacity constraints, chromatography columns continue to be…
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Q&As with Industry Leaders
Jeanne Linke Northrop, BioPharm International
Nora Ketterer, Manager, Modeling Services, Cytiva
Top Tips for Successful Development of Antibody Chromatography Processes
Jeanne L…
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Addressing the Challenges in Downstream Processing Today and Tomorrow
Weak partitioning chromatography (WPC) and a high throughput screening (HTS) tools have been essential for development of a robust two-column platform purification process. The benefits of a two-colum…
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Updating Viral Clearance for New Biologic Modalities
Thus, for example, one reduces the HCPs generated by a cell line by manipulating the buffers and chromatography chemistries to separate the product. In such cases, viruses are absent, and their presen…
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Impurity Testing of Biologic Drug Products
Physicochemical methods can be largely based on compendial procedures and require little development, and parameters for platform techniques such as size-exclusion high-performance liquid chromatograp…
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Preclinical Evaluation of Product Related Impurities and Variants
Obtaining the commercial GCSF formulation requires a series of bioprocessing steps including refolding and chromatography. Often, these processing steps result in formation of certain molecular varian…
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Modeling and Miniaturization: Key Topics at Successful HTPD Conference
HTPD was mainly limited to 96-well plates filled with chromatography resins used for resin selection and initial screening of running condition. Since then, HTPD in chromatography applications has bee…
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Challenges of Protein Aggregation During Purification
Different combinations of unit operations may be used to recover and purify a given product, including centrifugation, various types of chromatography (e.g., affinity, ion exchange, hydrophobic, etc.)…
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Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
Host cell proteins were removed using a ReadyToProcess Capto Core 700 chromatography column.
Capto Core 700 chromatography medium (resin) was used in the second chromatography step of the purifica…