Search results for " concentration" in Articles / App Notes
Article
The Development and Application of a Monoclonal Antibody Purification Platform
After anion exchange, the flow-through fraction containing the antibody was passed through a nanofiltration unit to remove potential viruses and then formulated in buffer in a final concentration and …
Article
Address the complexity of protein characterization
This presentation includes some examples on the use of Biacore™ surface plasmon resonance (SPR) systems for active concentration measurement, target binding, and describe how you can use these assays …
Article
Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
This video presents a study of the effects of different buffer substances, mono-, di-, and tri-carboxylic acids, and concentrations on elution pH, resolution between hetero- and homodimers for bsAb’s,…
Article
From Bottlenecks to Breakthroughs: Mastering Buffer Management
Unlock innovation in manufacturing by optimizing your buffer production.
Agenda
- Options to intensify buffer preparation from using pre-made buffers or buffer concentrates to impl…
Article
Evaluating the Use of Continuous Chromatography
In Cytiva’s case, its system can adjust accordingly to changes in feed concentration, for example, to optimize processing of the perfusion output. Also, pre-packed columns have provided greater consis…
Article
Exosome isolation by tangential flow filtration and size exclusion chromatography
The workflows we describe are suitable for scale-up and combine tangential flow filtration (TFF) for the concentration of exosomes followed by gentle size exclusion chromatography (SEC) with our next-…
Article
Report from the 6th International HTPD Conference
Several analytical assays for AAV require high concentration of viral particles and therefore requires large volumes of starting material, which is not always available during process development.
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Article
Report from the 12th Plasma Product Biotechnology Forum
The model output was a prediction of yield and purification rate, and the input included feed flowrate, conductivity, column loading, and total protein concentration. One of the conclusions from the s…
Article
Automated Buffer Preparation with Inline Conditioning Technology
Inline conditioning (IC) technology allows for buffers to be produced from single-component stock concentrates when needed, at the point-of-use (POU). IC automates buffer production, s…
Article
Webcast: Repeatable, automated SPR titer assay for adeno- and adeno-associated viruses in crude samples
Viral vectors are commonly used in clinical trials to evaluate gene therapies. Due to instability, these vectors are difficult to produce and analyze. Current assays lack the sensitivity requi…