Search results for " media" in Articles / App Notes
Article
Development of Purification for Challenging Fc-Fusion Proteins
The main parameters to consider when selecting HIC media and conditions are ligand hydrophobicity, salt type, and salt concentration. The feed stream was sourced from the first polishing chromatograph…
Article
Evaluation of HEK293 Cell Growth and Adenovirus Productivity in HyClone™ CDM4HEK293 Medium
Human embryonic kidney 293 (HEK293) cells are widely used in production of recombinant viruses and proteins because of their stable growth, high transfection level, and ability to produce high viral t…
Article
Process Chromatography: Continuous Optimization
Even so, there are many impurities present in the harvest cell-culture fluid obtained after removal of the cells or yeast and media. Host-cell proteins, host-cell DNA, other process-related contaminan…
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N-Glycan Analysis of Biotherapeutic Proteins
The presence of high-mannose glycans results in increased clearance mediated by mannose receptors (14), and as such, when ADCC constitutes the mechanism of action, the presence of high-mannose-type N-…
Article
Downstream Processing for Cell-Based Therapies
…carriers, culture surfaces, colonies, or embryoid bodies), these are generally quenched with culture media to preserve cell health by preventing the enzyme from further digesting cell components. Mor…
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Speed and Flexibility Are Dual Goals for Biopharma Operations
What new technologies and products are impacting bioprocessing operations?
Recent product introductions for bioprocessing operations—such as advances in bioreactor technology, media, buffers, and …
Article
High-Throughput Process Development Handbook
Time-to-clinic and time-to-market are two key factors for successful biopharmaceutical development. Efficient development of the manufacturing process is a crucial component of the overall project…
Article
Generating a Fully Processed Antibody
Also recently, the FGE enzyme was shown to require copper (II) for full activity (6, 7), so supplementation of copper (II) sulfate to the media optimizes amino acid conversion.
Cell-line engineeri…
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Affinity chromatography Handbook - Vol. 3: Specific Groups of Biomolecules
This volume of the Affinity Handbook describes the role of AC in the purification of specific groups of biomolecules, the principle of the technique, the chromatography media available and how to sele…
Article
Managing Biomanufacturing Capacity Expectations
Despite these benefits, concentrated fed-batch used more perfusion and feed media, required numerous filters, and also overloaded downstream processes, causing filter fouling in some cases. The yields…