Search results for " 96-well" in Articles / App Notes
Article
Development of Purification for Challenging Fc-Fusion Proteins
In this study, utilizing a combination of high-throughput condition screening in 96-well plates, optimization in small-scale column models, and a novel virus-spiking strategy, the authors have develop…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
An example illustrating the use of HTPD and DoE to optimize the first polishing step for a mAb of interest is discussed herein, and the roles of both HTPD 96-well filter plates and mini-columns are hi…
Article
Report from the 6th International HTPD Conference
HTPD was mainly limited to 96-well plates filled with chromatography resins used for resin selection and initial screening of running conditions. Since then, HTPD in chromatography applications has be…
Article
Miniaturization to Become Even More Successful
Several talks focused on the use of miniaturized columns and screening plates, such as PreDictor 96-well plates and PreDictor RoboColumn™ units, and the possibility of these formats to become even mor…
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Modeling and Miniaturization: Key Topics at Successful HTPD Conference
HTPD was mainly limited to 96-well plates filled with chromatography resins used for resin selection and initial screening of running condition. Since then, HTPD in chromatography applications has bee…
Article
High-Throughput Process Development Handbook
Evaluating chromatographic conditions can be performed in parallel using 96-well filter plates. As a result, a large number of experimental conditions can be evaluated simultaneously. This allows a la…
Article
Developing Cleaning-in-Place Protocols
Cleaning conditions for chromatography media can be screened in a couple of working days using 96-well microtitre plate formats. A study with a Protein A resin illustrates the approach in this appli…
Article
Top Tips for Successful Development of Antibody Chromatography Processes
So for instance, PreDictor™ plates, plates, a 96-well format, where you can screen 96 different conditions in the same experiment. There are also small, miniature columns that can be utilized in the s…
Article
A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
This method can also be adapted to a 96-well plate for high-throughput media screening. Validation of the assay for quantitation of glucose in the culture media includes the demonstration of specifici…
Article
Resolving a bottleneck in screening and characterization of recombinant antibody fragments using Biacore 4000
Ten 96-well plates containing avian scFv fragments in crude cell lysates were screened and ranked in less than 20 h. A subset from this screen was selected for analysis with the 2-over-2 kinetics appr…