Search results for " yield" in Articles / App Notes
Article
N-Glycan Analysis of Biotherapeutic Proteins
…osaccharide constituents with acid hydrolysis and separating the monosaccharides with HPAE-PAD (46), yielding information on the relative amounts of each monosaccharide. Other techniques for monosacc…
Article
Unifying Continuous Biomanufacturing Operations
The success of a truly integrated continuous processing platform relies on the collaborative efforts of upstream and downstream specialists.
By Randi Hernandez
When human cells travel throug…
Article
Fusion Proteins Pose Manufacturability Challenges
For example, the low-pH elution step used in the initial protein Acapture step may induce large amounts of aggregation, impacting product quality and yields. “Frequently, routine cell-culture and puri…
Article
Improvements in Protein A Chromatography
You mention that you sometimes are asked “Why do you still use Protein A?” Why do you?
Protein A chromatography provides high yield while providing up to 3 logs of host cell protein removal and 4…
Article
Mechanistic Modeling of Preparative Ion-Exchange Chromatography
Step 5: Process optimization
Once the model and model parameters are established, process optimization studies can be performed to achieve optimal selectivity and yield. The typical values for…
Article
Accelerated flavivirus vaccine production with modern tools and solutions
This white paper includes a Fast Trak Services case study on the use of such resins to increase purity and yield of a flavivirus vaccine manufacturing process.
Download white paper.
Article
Ensuring the Biological Integrity of Raw Materials
They can alter the metabolism and properties of cells and change product yield, cause false assay results, and generally wreak havoc in the culture.
Viruses are some of the simplest of all organis…
Article
Efficient Purification of the Pertussis Antigens Toxin, Filamentous Haemagglutinin, and Pertactin in Chromatography Workflows
The described method resulted in higher purity and yield than what can be expected from a purification process based on salt precipitations and sucrose gradient centrifugation.
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Article
Efficient Purification of Pneumococcal Polysaccharides in a Chromatography Workflow
…l polysaccharides to eliminate the need for phenol extraction, while increasing process recovery and yield.
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Article
A Platform Approach to Purification of Antibody Fragments
The described BioProcess™ resins offer high selectivity and excellent pressure-flow properties for high purity and yield in industrial-scale purifications. In addition, several case studies on purific…