Search results for " buffers" in Articles / App Notes
Article
Best Practices in Qualification of Single-Use Systems
The author discusses the current best practices in technical qualification of single-use systems.
By Weibing Ding, PhD
Single-use technology has been around for approximately two decades if …
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
The issues with dilution have not been observed for buffers and salt containing aqueous solutions.
Optimizing binding capacity
The dynamic binding capacity is dependent upon several experiment…
Article
Re-use of Protein A Resin: Fouling and Economics
Mar 01, 2015
By Anurag S. Rathore, Mili Pathak, Guijun Ma, Daniel G. Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography ha…
Article
Detecting Protein Aggregates and Evaluating their Immunogenicity
Mar 01, 2015
By Randi Hernandez
BioPharm International
Volume 3, Issue 28
Aggregation in biopharmaceuticals remains a major concern and threatens the stability of a product. Protein …
Article
SEC in the Modern Downstream Purification Process
Mar 01, 2015
By R. Christopher Manzari, J. Kevin O'Donnell
BioPharm International
Volume 3, Issue 28
Since the introduction of commercial chromatography resins approximately six de…
Article
Analyzing Proteins Using SEC, MALS, and UHPLC
The range of columns and buffers available for use in SEC, however, means that there are always improvements to be made in method development, according to Pothecary. “Alternative columns, different …
Article
Under pressure: exploring root causes of leaks in single-use mixers
Single-use technology offers huge benefits for both flexibility and efficiency. It reduces downtime by eliminating cleaning steps, helps avoid risk of cross-contamination, and enables modulari…
Article
Advances in Sterilization Technologies for Overcoming Viral-Vector Manufacturing Challenges
Sterility must be assured for all biologic drug products. Terminal sterilization achieved via treatment with heat, radiation, or certain chemicals (vaporous hydrogen peroxide, vaporous peracet…
Article
FAQs about Cytiva™ Protein Select™ technology
Cytiva™ Protein Select™ resin is an affinity chromatography resin for purifying any recombinant protein that does not have an affinity binding partner.
Purifying a protein tagged with thi…
Article
Webinar about a new affinity chromatography technology for recombinant proteins
Introducing new Cytiva™ Protein Select™ resin for recombinant protein purification in research and process development
On November 15, 2023, Cytiva announced the upcoming l…