Search results for " buffers"
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Implementing Inline Conditioning to Advance Process Intensification
With inline conditioning buffers can be prepared inline from buffer concentrates or stock solutions, and directly integrated with the chromatography or filtration step. This eliminates the need for in…
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Modeling the Degradation of mAb Therapeutics
In brief, buffers examined included those commonly used for Protein A chromatography, cation exchange chromatography, and anion exchange chromatography.
Samples were kept at three different te…
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Improving Process-Scale Chromatography
In-line concentration solutions
A number of technologies have been introduced to help address the challenges associated with the preparation and storage of the large volumes of different buffers r…
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What’s New in Upstream Technologies
Wet, one-point standardization before startup allows for the sensor’s characteristics and functionality to be verified before any solutions or buffers are added to the bioreactor bag. The standardizat…
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Development of Purification for Challenging Fc-Fusion Proteins
Equilibration with five-column volume was performed followed by varying the load-ratio conditions and chase with equilibration buffers. Fractions of the flow-through were collected during the loading…
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Making the Move to Continuous Chromatography
The manufacturer just needs the right equipment and expertise; the processes use the same buffers and resins as used in their current batch processes,” he says. “Ultimately, if you want to work faster…
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Fluid Handling in Biopharma Facilities
BioPharm: Which fluids represent the largest volumes within a facility?
Blomberg (Meissner): Typically within a biopharmaceutical manufacturing process that employs single-use systems, buffers and …
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Other advantages of developing a method using a quaternary solvent system includes ease of mobile phase composition control, which can greatly reduce the labor needed to make and change a variety of b…
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QbD and PAT in Upstream and Downstream Processing
Control of these critical buffers is managed through batch preparation and release prior to use based on acceptable pH and conductivity ranges. Process capability is sufficient to reproducibly prepare…
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Single-Use for Downstream Chromatography: Benefit or Hindrance?
Prefilled BPCs have only made the single-use option more popular, he says, noting that BPCs prefilled with water for injection or purification buffers can be delivered to customers, eliminating the ne…