Search results for "cho" in Articles / App Notes
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
By Luhong He, Christopher Frye
Abstract
The Chinese hamster ovary (CHO) cell lines used for the production of recombinant therapeutic proteins are immortalized cells with a relatively high degre…
Article
Optimization of Fed‑Batch Culture Conditions for a mAb‑Producing CHO Cell Line
This application note describes a broadly applicable and efficient workflow toward finding the optimal feed combination for the best-performing fed-batch processes.
An IgG1-producing CHO cell …
Article
Impact of Media Components on CQAs of Monoclonal Antibodies
The choice of cell culture media has been known to significantly affect the physiochemical characteristics of mAbs. The selection of the media depends on the type of cells to be cultured and also the …
Article
Platform Technologies Improve Protein Expression
…icians, and ultimately patients in need of novel medications,” notes Bjørn Voldborg, director of CHO cell-line development at the Novo Nordisk Foundation Center for Biosustainability.
Improving…
Article
Generating a Fully Processed Antibody
…other additional post-translational modifications not typically performed by Chinese hamster ovary (CHO) cells, and more recently, numerous different multispecific antibody platforms. Each of these c…
Article
Platform Approach Speeds Process Development
Chinese hamster ovary (CHO)-based cell culture platforms should combine state-of the-art technologies for genetic vectors, host cell portfolio, cell line generation, cultivation media, and process des…
Article
N-Glycan Analysis of Biotherapeutic Proteins
For example, arguably the most common N-glycan structure present on mAbs made in Chinese hamster ovary (CHO) cells is G0F: asialo-, agalacto-, biantennary complex N-glycan, core-substituted with fucos…
Article
State of the Biopharmaceutical Industry: Lessons from 2018 Survey Data
Despite diverse genetic-engineering methods and host organisms being available, industry has clearly come to prefer and standardize on use of mammalian systems, particularly CHO. Many more efficient o…
Article
Implementation of Raw Material Control Strategies in the Manufacture of Single-Use Bioprocessing Containers
One of the degradation products, bis(2,4-di-tert-butylphenyl)phosphate (bDtBPP), inhibits growth of some Chinese hamster ovary (CHO) cell lines (8, 9, 10).
A stabilizer-degradation product impacti…
Article
Leveraging Computational Models of Glycosylation for Biopharma QA
Large-scale mammalian cell culture is used for the manufacture of all therapeutic glycoproteins (TGPs), with Chinese hamster ovary (CHO) and murine myeloma (NS0 and Sp2/0) cell lines being the most-co…