Search results for "cho"
Article
Biopharma in 2016: Higher Quality Drugs, Less Expensive Manufacturing
…wnstream Process Intensification
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
A Second Modular Biopharma Facil…
Article
Modeling Bioreactor Performance
“The real work is building the model within whichever tool is chosen,” he states.
Meeting modeling challenges
Challenges to successful modeling come from technical and resource limitations. On…
Article
Safety Drives Innovation in Animal-Component-Free Cell-Culture Media Technology
But because the chosen methods focused primarily on downstream virus removal or inactivation instead of strategic sourcing of lower-risk starting materials, the efforts had little effect on serum-free…
Article
A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
…five different chemically defined components of cell-culture media used in a Chinese hamster ovary (CHO) cell manufacturing process for recombinant proteins (11). Although, both NIR and Raman spectro…
Article
Continuous Processing for the Production of Biopharmaceuticals
The high-density Chinese hamster ovary (CHO) cells were operated at a quasi-steady stage for more than 60 days. The PCC system was directly integrated into this and ran continuously for 30 days. Produ…
Article
Antibody Production in Microbial Hosts
Mammalian cells have been primarily applied as the hosts of choice for mAb expression. However, clinical success of mAbs has tremendously increased their demand, and this in turn has fueled interest i…
Poster
One-step seed culture expansion from one vial of high-density cell bank to 2000 L production bioreactor
This application note describes how perfusion cell culturing can be used to reduce processing time, simplify operations, and maximize equipment utilization in seed culture expansion processes.
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
Case study: Accelerating mAb purification via HTPD
The mAb used in the following case study was produced in Chinese hamster ovary (CHO) cells, and the initial mAb capture step was carried out with a…
Article
Host-Cell Protein Measurement and Control
coli) has ~4300 genes, whereas Chinese Hamster Ovary (CHO) cells have ~30,000 genes (1). Although not every host gene will be transcribed and translated to protein, the complexity of host genome and t…
Article
Use of Multivariate Data Analysis in Bioprocessing
Researchers have monitored performance of mammalian (Chinese hamster ovary [CHO]-based) bioprocesses using raman spectroscopy (14). The authors used MVDA algorithms such as Competitive adaptive reweig…