Search results for "cho"
Article
Platform Approach Speeds Process Development
Chinese hamster ovary (CHO)-based cell culture platforms should combine state-of the-art technologies for genetic vectors, host cell portfolio, cell line generation, cultivation media, and process des…
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N-Glycan Analysis of Biotherapeutic Proteins
For example, arguably the most common N-glycan structure present on mAbs made in Chinese hamster ovary (CHO) cells is G0F: asialo-, agalacto-, biantennary complex N-glycan, core-substituted with fucos…
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State of the Biopharmaceutical Industry: Lessons from 2018 Survey Data
Despite diverse genetic-engineering methods and host organisms being available, industry has clearly come to prefer and standardize on use of mammalian systems, particularly CHO. Many more efficient o…
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Implementation of Raw Material Control Strategies in the Manufacture of Single-Use Bioprocessing Containers
One of the degradation products, bis(2,4-di-tert-butylphenyl)phosphate (bDtBPP), inhibits growth of some Chinese hamster ovary (CHO) cell lines (8, 9, 10).
A stabilizer-degradation product impacti…
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Leveraging Computational Models of Glycosylation for Biopharma QA
Large-scale mammalian cell culture is used for the manufacture of all therapeutic glycoproteins (TGPs), with Chinese hamster ovary (CHO) and murine myeloma (NS0 and Sp2/0) cell lines being the most-co…
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Development of Purification for Challenging Fc-Fusion Proteins
…electric point (pI) ranging from four to seven were expressed in recombinant Chinese hamster ovary (CHO) cells and partially purified through ProA capture chromatography. The load for the Fc-fusion P…
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Simple Fed-Batch Process Development for a Knockout Cell Line
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This workflow improved titers by 500% compared with Chinese hamster ovary cell culture in basal medium.
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News: Cytiva Adds ELISA Kit to End-to-End HCP Monitoring Capabilities
Cytiva is pleased to announce the new Amersham HCPQuant CHO kit, a generic for detecting host cell protein impurities in drug samples produced in CHO cell lines.
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Perfusion Culture Using TFF or ATF as Cell Retention Method
The work demonstrates perfusion culture of an antibody producing Chinese hamster ovary (CHO) cell line, using either tangential flow filtration (TFF) or alternating tangential flow filtration (ATF) as…
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Optimizing Late-Stage and Commercial Cell-Culture Processes
Beyond that, Racher says, there is increased incorporation of synthetic biology paradigms into Chinese hamster ovary (CHO)-based expression platforms, moving the industry beyond simply increased titer…