Search results for " capture" in Articles / App Notes
Article
Automated In-Line Buffer Preparation from Ready-Made Stock Solutions in a mAb Process Step
Buffers of different formulations for a mAb chromatography capture step were prepared in an automated, consecutive manner, minimizing manual interactions. To further reduce the time and space required…
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Fusion Proteins Pose Manufacturability Challenges
Finally, Chamow adds that Protein A capture chromatography can present problems for fusion proteins, not only with respect to their acid stability, but also in terms of lower binding capacities due to…
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Downstream Processing for Cell-Based Therapies
Acoustic filtration uses standing waves to capture, separate, and concentrate particles in a fluid without having a physical barrier (membrane or filter) like traditional filtration systems. It is, th…
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Mechanistic Modeling of Preparative Ion-Exchange Chromatography
The system captures the extra column volume as well as the dispersion effect of the system, thus allowing the measurement of the accutate protein and salt profile that enters and leave the column.
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Innovation vs. Capacity: How CMOs Compete
Having well-thought-out strategies that target more attractive market segments, align capabilities to serve those segments, and capture market share are more crucial than ever for achieving healthy re…
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Optimize Column Loading Strategy to Gain Productivity in Protein Purification
Are you looking for a way to improve productivity in your affinity chromatography step? Have you ever applied the concept of variable loading to gain productivity? By varying residence time (RT) ove…
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Reporting Quality Metrics to FDA
Since the publication of FDA’s draft guidance, Request for Quality Metrics, Guidance for Industry (1), in July 2015, we have been debating how to best capture and report quality metrics in our organiz…
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Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
Details about the nature of the process also need to be captured, including the bio-safety level and lengths of culture time for the seed and production bioreactors.
Moving to downstream, the over…
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Resolving a bottleneck in screening and characterization of recombinant antibody fragments using Biacore 4000
Chimeric chicken/human Fab fragments were screened and characterized using a preoptimized Human Fab Capture Kit to achieve accurate off-rate determination during initial screening of crude samples. Th…
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Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
This involves the immobilization or capture of the different antigens, followed by calibration-free concentration analysis (CFCA) analysis. Because bispecific molecules are used as analytes, the ratio…