Search results for " capture"
Article
Process Chromatography: Continuous Optimization
…ly purified using a platform approach in which protein A chromatography resins are used in the first capture step. Polishing is then achieved using some combination of anion exchange (AEX), cation ex…
Article
Unifying Continuous Biomanufacturing Operations
They added that this so-called “culture and capture” approach is expected to “reduce heterogeneity in other product characteristics such as glycation or oxidation with respect to classical batch opera…
Article
Improvements in Protein A Chromatography
Alternative technologies like CEX capture or precipitation often provide lower purity and require more mAb-specific development.
What future directions would you like to see in the development o…
Article
Host-Cell Protein Measurement and Control
In Figure 1, capture antibodies enrich the HCPs from sample and immobilize them to a 96 (or greater)-well plate. Then, detection antibodies, conjugated directly with an enzyme or through a biotin-avid…
Article
Detecting Protein Aggregates and Evaluating their Immunogenicity
LO alone is not sufficient to detect aggregates, as it is not sensitive enough to always capture translucent particles. Aggregation can cause protein particles to become transparent, and relying solel…
Article
Comparing Protein A Resins for Monoclonal Antibody Purification
Protein A is the affinity chromatography ligand of choice for the first antibody capture step, because its high selectivity gives excellent purity (typically > 99%) and high yields. Furthermore, Prote…
Article
Evaluating the Use of Continuous Chromatography
…an overload the first column [because] any product/target molecule that is breaking through is being captured by the second column in the loading zone. When used with a perfusion or fed-batch bioreac…
Article
A Closer Look at Affinity Ligands
…ioCina): Affinity ligands can produce a very high purification factor in a single unit operation and capture product from a dilute process stream. This makes affinity purification very attractive in …
Article
Top Tips for Successful Development of Antibody Chromatography Processes
But working with protein L, that only binds kappa subclass one, three and four, for instance, MabSelect™ VL, you could get a really high purity sample during the capture step. Since the lambda heterod…
Article
Chromatography innovations for the diverse antibody pipeline
In this video Josefin Bolik, global product manager — antibody capture at Cytiva, presents how to select resins for your antibody variant purification project.
WATCH THE VIDEO >>