Search results for " capture"
Article
Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
Details about the nature of the process also need to be captured, including the bio-safety level and lengths of culture time for the seed and production bioreactors.
Moving to downstream, the over…
Article
Resolving a bottleneck in screening and characterization of recombinant antibody fragments using Biacore 4000
Chimeric chicken/human Fab fragments were screened and characterized using a preoptimized Human Fab Capture Kit to achieve accurate off-rate determination during initial screening of crude samples. Th…
Poster
Optimizing productivity on high capacity protein A affinity medium
MabSelect SuRe™ protein A affinity medium has long been considered a starndard tool for capturing monoclonal antibodies in biopharmaceutical production. This application note summarizes studies on d…
Article
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
This involves the immobilization or capture of the different antigens, followed by calibration-free concentration analysis (CFCA) analysis. Because bispecific molecules are used as analytes, the ratio…
Poster
Purification platform challenges and strategies for handling of "tricky" MAbs
In the work presented here, capture using high-capacity protein A medium (resin) was followed by polishing using a novel cation exchange chromatography medium.
Article
Risk Assessment and Mitigation in Biopharmaceutical Manufacturing
Management reviews need to capture and aggregate risks across manufacturing campaigns to mitigate potential risks during changeover. In general, a biopharmaceutical manufacturer faces similar business…
Poster
Three-step monoclonal antibody purification processes using modern chromatography media
This application note describes monoclonal antibody (MAb) purification processes using the expanded mAb purification toolbox of Cytiva business, covering modern chromatography media (resins) for sta…
Article
Advanced Technologies Facilitate Scale-up and Technology Transfer
As one example of how Fujifilm is using technology to address current challenges, Bird cites the company’s “TAG” system for digitally managing the capture, conveyance, and retention of manufacturing k…
Article
Biomanufacturing Outsourcing Globalization Continues
Conclusion
CMOs and contract research organizations based in emerging markets will continue to capture market share, albeit slowly. These CMOs continue to face domestic regulatory and legal hurd…
Article
Optimization of Protein Expression in Escherichia Coli
For example, for proteins synthesized with targeting signal sequences, the tag should be located at the C-terminus to avoid mis-targeting and to ensure mature protein capture, especially if the signal…