Search results for " capture"
Article
A Look into the Future of Biopharmaceutical Quality
By the time updates are made to the pharmacopeias to capture the current technological advancements, more sophisticated equipment and methodologies are being introduced.
The industry seems to b…
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Integrated Semi-Continuous Process for mAb Production
In brief, the mAb process consists of perfusion cell culture, continuous capture chromatography, viral inactivation (VI), post VI filtration, batch polishing chromatography, and a final pH adjustment …
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Selection Guide: Columns and Resins for Antibody Purification and Immunoprecipitation
Polyclonal antibodies, monoclonal antibodies (mAbs), and antibody fragments are usually purified by affinity chromatography. Resins containing an immobilized ligand (e.g., protein A, protein G, or p…
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Cleaning Protein A Resins with 1 M NaOH
The presence of cell culture nutrients in the harvest feed loaded on the capture column also exposes the protein A step to increased risk of microbial contamination. Historically, protein A resins hav…
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Applying GMPs in Stages of Development
It also helps to capture the product and process history needed in the later phases of development for the process validation activities.
To determine the impact of the deviation on the …
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Navigating Data Integrity in the Modern Lab
The technology of the past decade has allowed for better preservation of data, from the time of capture in electronic format through to its analysis and use in reports.
This digital thre…
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Preclinical Evaluation of Product Related Impurities and Variants
…t the number of fractions used in the analysis represents sufficient variability that is adequate to capture the impact of the variants on the activity. Second, keeping roughly the same amounts of ac…
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Reducing Cross-Contamination Risks in Process Chromatography
Bulpin notes that single-use chromatography affinity solutions will enable rapid cycling chromatography and continuous capture at high speed. To address the industry need for continuous processing, Mi…
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Modeling and Miniaturization: Key Topics at Successful HTPD Conference
Walther presented a miniaturized and parallelized downstream process consisting of mechanical cell disruption, IB wash, protein renaturation, and capture chromatography carried out in 96-well plates, …
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Automated In-Line Buffer Preparation from Ready-Made Stock Solutions in a mAb Process Step
Buffers of different formulations for a mAb chromatography capture step were prepared in an automated, consecutive manner, minimizing manual interactions. To further reduce the time and space required…