Search results for " low pH" in Articles / App Notes
Article
Antibody Production in Microbial Hosts
The authors review the status of expression of antibodies in microbial hosts and present the recent advances in the production of aglycosylated antibodies in bacteria.
By Anurag S. Rathore, Jyot…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
Optimization of each phase in a chromatographic cycle has a positive impact on productivity.
By Anna Grönberg
An important area of focus for the biopharma industry is the elucidation and dev…
Article
Re-use of Protein A Resin: Fouling and Economics
…s from the harvest; wash step with the selective buffer to remove non-binding impurities; elution at low pH and conductivity; and finally, cleaning and sanitization of the column for next use. The wi…
Article
Application of Quality by Design to Viral Safety
In a low pH hold designed for viral inactivation, for example, a lower pH and longer hold time than some critical values would provide robust inactivation and would be included in the viral clearance …
Article
The Development and Application of a Monoclonal Antibody Purification Platform
The eluate was then held at this low pH to inactivate viruses, and then adjusted to increase the pH before the cation exchange chromatography step.
After cation exchange, diafiltration was perf…
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
If used correctly, these new analytical methods can reduce analysis and product development time.
ABSTRACT
Monoclonal antibodies represent a significant portion of sales in the biopharma…
Article
Dear lentivirus: We have not forgotten about you and how to improve your polishing recovery
Vector purification is very challenging due to low stability of these enveloped viruses, which are sensitive to low pH, high salt, temperature, shear forces, and other factors.
To maximize physica…
Article
Enhancing Resins Addresses Purification Concerns
Christoph Burgstedt/Stock.Adobe.com
Enhancement of resin technology can improve the ability of chromatography to purify the growing landscape of increasingly complex biomolecu…
Article
Innovative Chromatography Resins Can Improve Purity and Quality
By: Feliza Mirasol
Recent innovations in chromatography resins offer promising advantages in downstream bioprocessing.
With ongoing efforts to increase monoclonal antibody (mAb) productivi…
Article
Finding the Needle in a High-Dimensional, Information-Rich Data Haystack Can Be Time Consuming
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Biacore™ surface plasmon resonance (SPR)-based sensors are an essential tool in drug discovery. With these tools, scientists can detect and characterize low molecular …