Search results for " techniques"
Article
Affinity chromatography Handbook - Vol. 3: Specific Groups of Biomolecules
Affinity chromatography (AC) separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. The techni…
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A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
Several spectroscopic non-destructive techniques, including Fourier Transform Infrared (FT–IR), near-infrared (NIR), and Raman spectroscopy have been used for the identification and characterization o…
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Antibody Production in Microbial Hosts
Though substantial progress has been made in cellular engineering and in the secretion pathway of microbial cells, further progress in metabolomic and proteomic techniques is required to improve the u…
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Selecting a Comprehensive Bioburden Reduction Plan
Personnel Training
BioPharm: Does operating personnel need specific training on bioburden reduction?
Souza: The manufacture of biologics requires operators trained in aseptic techniques, with …
Article
Use of Multivariate Data Analysis in Bioprocessing
Of a variety of approaches, use of advanced sampling techniques, new sensor technologies, and analyzers has emerged as a topic of interest to the scientific community at large (2). Implementation of t…
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Quality by Design and Extractable and Leachable Testing
These extracted chemicals are then identified by various analytical techniques, typically inductively coupled plasma mass spectrometry (ICP—MS), liquid chromatography-mass spectrometry (HPLC–MS), and …
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Ligand-Binding Assays and the Determination of Biosimilarity
LBA detection techniques
There are essentially two general methods for assessing ligand binding: ELISA (enzyme-linked immunosorbent assay)-type assays and kinetic binding assays. Classical ELISA a…
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What Drove Biopharma Development in 2014?
According to a 2014 CPhI report, more than 70 percent of pharma manufacturers are looking to increase quality and invest in technologies and techniques like QbD to achieve that goal. And while QbD may…
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DoE Provides Benefits, but Preparation Is Necessary
Consequently, reaping the benefits requires a significant commitment to DoE, including the training of researchers in study design and data-processing techniques.
The benefits of DoE
One of th…
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Analyzing Proteins Using SEC, MALS, and UHPLC
The characterization of proteins involves identification of their complex chemical and physical structures and requires the use of a range of analytical techniques. Size exclusion chromatography (SEC)…