Search results for " mammalian" in Articles / App Notes

Article Analysis of Glycosylation in Biosimilars
The mammalian cells that are typically used for the culture can produce up to four ‘arms,’, or tetra-antennary structures. These structures can be seen at the high mass (right) side of the upper mass …

Article Managing Biomanufacturing Capacity Expectations
According to numbers from the 2016 BioPlan report (1), approximately 59% of respondents used at least some outsourced capacity for mammalian cell culture, 55% used outsourcing for microbial fermentati…

Article Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
Will the facility be manufacturing monoclonal antibodies, recombinant proteins, vaccines, antibody-drug conjugates, or fragment antibodies? Also, will the products be mammalian cell-derived or microbi…

Article A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Evaluation of integrity of the transgene mRNA expressed in CHO cells RNA splicing is a natural process in mammalian cells that removes introns and joins exons in a primary transcript to create matu…

Article Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
A typical bioprocess for a mammalian cell line may have 170 process factors. Using this approach, a 170-dimensional space would require 3.3 x 10119 cultures for full characterization, which is not fea…

Article Best Practices in Qualification of Single-Use Systems
Vinci and S Parekh, Handbook of Industrial Cell Culture: Mammalian, Microbial and Plant Cells (Humana Press, 2003). 13. P. Culter, Protein Purification Protocols, Second Edition (Humana Press, 200…

Article Biopharma Advances Demand Specialized Expertise
…hat are the roadblocks to implementation? Lorimer (Patheon Biologics): Continuous production from mammalian cell cultures has been operated in perfusion bioreactors for many years. The main roadbl…

Article Host-Cell Protein Measurement and Control
Although not every host gene will be transcribed and translated to protein, the complexity of host genome and the post-translational modification present in mammalian cells make it almost impossible t…

Article Vaccine Development and Production Challenges Manufacturers
Novartis’ vaccine plant in Holly Springs, NC was recently licensed to produce Flucelvax using mammalian cell culture. But the plant’s future is murky following Novartis’ divestiture of most of its vac…

Article Improving PAT for Biologics
Beri, head of mammalian research and technology for Lonza Custom Development and Manufacturing. In addition, PAT is much more than just one sensor integrated into a process. “Effective PAT implementat…

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