Search results for "Protein"
Article
Enhancing Resins Addresses Purification Concerns
What COVID-19 brings to the table
The COVID-19 vaccines comprise several possible and different modalities, such as messenger RNA (mRNA), attenuated viruses, viral vectors, proteins, and virus l…
Article
Cytiva Advances Fiber Chromatography Technology with New Launch
The product complements HiTrap Fibro PrismA, the company’s new fiber-bases Protein A platform launched for research applications in 2020.
The Fibro technology features an open porous adsorbent mat…
Article
Updating Viral Clearance for New Biologic Modalities
Because viruses are typically smaller than downstream impurities such as host cell proteins (HCPs) and other extracellular debris, they require meticulous filtration, even in more established processe…
Article
Western Blot Detection: Is Film or a Digital CCD Imager Better?
It is based on building an antibody:protein complex via specific binding of antibodies to proteins immobilized on a membrane and detecting the bound antibody with one of several detection methods.
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Article
Using Multiple Techniques in Biosimilar Analysis
Proteins are dynamic molecules that do not exist in a single conformation, but as a complex distribution of an ensemble of different conformations. This dynamic conformational landscape will commonly …
Article
How to Determine Dynamic Binding Capacity (DBC) of Chromatography Resins
In some cases, you might need to determine the DBC yourself to screen for the best resin and running conditions for your specific protein.
Poster
Scale-up of CHO fed-batch cultures in HyClone™ ActiPro™ medium supplemented with Cell Boost™ 7a and 7b
Results from shake flask cultures show high viable cell densities and protein production. In addition, scalability of the culture process was demonstrated in 2 and 50 L bioreactor cultures. Cell densi…
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
Host cell proteins were removed using a ReadyToProcess Capto Core 700 chromatography column.
Capto Core 700 chromatography medium (resin) was used in the second chromatography step of the purifica…
Article
Detailed agenda for Chromatography + filtration virtual summit
Join Cytiva Chromatography and filtration summit online to learn from presentations, live Q&A, and panel discussions on recent advances in antibody, antibody variants, recombinant proteins, and gene t…
Article
The Importance of Process Intensification and PAT for Achieving Real-Time Release
Commercially available PAT solutions that will fuel the rise of intensified processing of therapeutic proteins are limited in number, however. Close collaboration between vendors, manufacturers, and h…