Search results for " bioreactor"

Article Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
First, the availability of high-throughput miniature bioreactors has made it possible to evaluate many more conditions. This has enabled design of experiment (DoE) approaches, such as response surface…

Article Best Practices in Qualification of Single-Use Systems
Aseptic connector devices, mixer bags, storage bags, and bioreactors followed. Currently, single-use systems (SUS) also include disconnectors, disposable filling needles, and sensors. The technology i…

Article Evaluating the Use of Continuous Chromatography
…h is a useful approach when the purification step is connected to an upstream perfusion or fed-batch bioreactor, and, by having two or more columns in the loading zone, you can overload the first col…

Article Simplify-bioreactor scale up and scale down: applications and tools
In this article we present the theory for bioreactor scaling and propose a workflow to scale the agitation and aeration regimes. READ ARTICLE >>

Article Best Practices for Selecting a Top-Quality Cell Line
Before scaling up to bioreactors, the productivity level for monoclonal antibodies (mAbs) should be 2–7 g/L (fed-batch culture in shake flask) or greater, observes Selexis CEO Igor Fisch. Once scaled …

Article Ensuring Viral Safety of Viral Vaccines and Vectors
Extraneous vesiviruses, however, have recently appeared in bioreactors (2–5), and porcine circovirus type 1 (PCV-1) contamination of oral rotavirus vaccines was first reported by a metagenomics analys…

Article Optimizing Late-Stage and Commercial Cell-Culture Processes
This resulted from the introduction of parental cell lines better suited to modern fed-batch processes and the development of better bioreactor processes, including control strategies for pH, carbon d…

Article Challenges in Cell Harvesting Prompt Enhancements
Biomanufacturers have been shifting from large stainless-steel bioreactors in the thousands-of-liters range to smaller, disposable bioreactors (2). The need to address this bottlenecking issue …

Article Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
For example, one can consider 2 x 2000-L bioreactors running a typical 14-day incubation period staggered a week apart, which equates to one batch produced each week. A typical batch at 2 g/L with a 7…

Article A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Generation 0 (G0) represents the generation of a master cell bank, G30 represents the generation of cells harvested from a 5000-L bioreactor, and G60 represents the limit for in vitro cell age designe…

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