Search results for "Upstream"
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
Increasing upstream titers can make the Protein A chromatography process step the rate-limiting step in downstream purification. Protein A chromatography columns are frequently too large, limiting the…
Article
Bioburden Control in the Biopharmaceutical Industry
…priate selection of pre-filters rating and risk assessment studies that should be assessed for both upstream and downstream processes.
Good Practices in the Biopharmaceutical Industry
Because…
Article
Cost Considerations Drive Lean Technology in Biopharmaceutical Manufacturing
Alternatively, upstream process scale-up can make use of perfusion reactors, which use a constant supply of cell-culture media while removing unwanted byproducts throughout a prolonged production run …
Article
Automating Bioprocesses
By Jennifer Markarian
Both upstream and downstream biopharmaceutical processes have conventionally used stainless-steel equipment, but use of single-use systems (SUS) is becoming more established.…
Article
Improving Process-Scale Chromatography
Cytiva conducted several lifecycle assessments (LCAs) for complete mAb production processes, upstream to downstream, comparing single-use stainless-steel approaches.
LCAs provide a holistic perspe…
Article
Process Chromatography Selection for Downstream Processing Applications
It is important to also consider that changes in the upstream steps may impact the useful lifetime of the resin.
The process performance, for example aggregate removal on a cation exchanger, may b…
Article
Managing Biomanufacturing Capacity Expectations
It is more difficult to respond to shifting demands if process equipment and clean utility systems are hard piped into the infrastructure, says Parrish Galliher, CTO for upstream at Cytiva. Multiple c…
Poster
Unlocking the potential for efficiency in downstream bioprocesses
Upstream processes are more efficient and product higher titers than before. With these improvements, new challenges arise. Downstream processes have to handle higher amounts of both targetprotein and…
Article
Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
Figure is courtesy of Cytiva
Starting with upstream, the status of the cell line and whether the process should be batch, fed-batch, or perfusion needs to be decided. Details about the nature of…
Article
Going Small to Achieve Success on the Commercial Scale
Scale-down models are used in the development of nearly every unit operation in the overall biopharmaceutical manufacturing process, including both upstream and downstream steps, and separation and fi…