Search results for "expression" in Articles / App Notes
Article
Determining Criticality–Process Parameters and Quality Attributes Part III: Process Control Strategies—Criticality throughout the Lifecycle
These mathematical expressions can be represented graphically as either contour or 3-D response surface plots.
Even this view of the design space is too simplistic. To ensure a process has a…
Article
Best Practices in Adopting Single-Use Systems
Bioprocessors must first analyze benefits and risks for their specific situation, keeping in mind their expression system, process, product, and corporate objectives.
After determining the suitabil…
Article
Trends in Downstream Bioprocessing
• Expression systems are producing 5 times what they were 10 years ago.
• Current downstream/purification areas were not designed to handle this increase in production.
• Buffer capacity, colu…
Article
Navigating Emerging Markets: Middle East and North Africa
The pharmaceutical industry grows despite conflict in the Middle East.
The Middle East and North Africa have been rocked in recent years by upheaval ranging from moderate protest to full…
Article
A Platform Approach for the Purification of Domain Antibodies (Dabs)
This Application note describes a three-step purification process of a domain antibody (Dab) expressed in the periplasm of E. coli. First, a capture step using Capto™ L was used to reduce E. coli host…
Article
The Future of Biopharma
… clinical and commercial scale
• continuous processing, including perfusion
• high-tech expression systems and other genetic engineering advances
• automation, monitoring, and process con…
Article
Quality by design for biotechnology products—part 1
…unogenicity, altering proteolytic stability, introducing chemical modification sites, and improving expression. Rational design approaches make it possible to identify and engineer better protein the…