Search results for " Fab" in Articles / App Notes
Article
Exploring the world of antibodies: mAbs, Fabs, dAbs, and bsAbs
There is a vast array of antibody variants, including multispecific (msAb), bispecific antibodies (bsAb), antibody fragments (Fab, dAb, scFv), and antibody-drug conjugates (ADC). But with this…
Article
Single-Domain Antibodies for Brain Targeting
IgG, the main serum antibody, the intact form of which is almost exclusively used in therapeutic antibodies, is a Y-shaped, multidomain protein with antigen-binding sites located on the two Fab (antig…
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Modeling the Degradation of mAb Therapeutics
Cleavage of the peptide bond (hydrolysis) at the mAb’s hinge region leads to the formation of Fc -Fab and Fab species (22).
Responses from different classes of the mAb molecules vary with resp…
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Antibody Production in Microbial Hosts
Figure 1: Structure of an antibody and its fragments: A) Antibody (IgG class); B) antibody fragments (VH, scFv, Fab, scFab, dAb, scFv-CH3). Abbreviations are: variable heavy chain (VH), variable light…
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Addressing the Challenges in Downstream Processing Today and Tomorrow
Cimzia and Lucentis are two recently approved Fabs produced in E. coli (29) and although no scFv has yet received regulatory approved, there are a large number of both types of Ab fragments in clinica…
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Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
Protein L resins bind to kappa light chain and are often used to purify antibody fragments such as dAbs and Fabs — but they can also be used to remove product-related impurities from bispecif…
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Guide for selecting affinity resins for mAbs, bsAbs, and antibody fragments
Today, molecules such as bispecific antibody (bsAb) and antibody fragments such as Fab, scFv, and VHH are seen in labs all over the world. These target molecules need to be purified to reach required …
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Chromatography innovations for the diversified antibody pipeline
MabSelect™ VH3 resins provides efficiency in capture of bispecific antibodies and antibody fragments such as Fab, scFv, and VHH. Discover how we’re supporting the diverse antibody pipelines with chrom…
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Protein L affinity resin is ready to move to manufacturing
After comparing resin performance for mAb, Fab, and dAb samples, MabSelect™ VL protein L resin offered increased binding capacities versus other resins. This improvement could address existing protein…
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
A major advantage of SPR-based analysis is its ability to estimate the association and dissociation rate constants, an …