Search results for " aggregation"
Article
A Q&A With Prof. Alois Jungbauer: The Role of HIC in Biotech
Process Development Forum speaks with Professor Alois Jungbauer, Department of Biotechnology, BOKU, Vienna, about Hydrophobic Interaction Chromatography (HIC).
What is your view of HIC in the bi…
Article
Risk Assessment and Mitigation in Biopharmaceutical Manufacturing
The challenges and strategies of assessing and mitigating risk in biopharmaceutical manufacturing are discussed.
Jul 01, 2015
By Susan Haigney
BioPharm International
Volume 28, Iss…
Article
Biopharma Advances Demand Specialized Expertise
Biosimilars have also caused the re-emergence of orthogonal techniques like size exclusion chromatography-multi-angle laser light scattering (SEC-MALL) and SV-AUC for aggregation and circular dichrois…
Article
Optimization of Protein Expression in Escherichia Coli
Many problems are associated with protein expression, including toxicity, misfolding and degradation, aggregation in inclusion bodies, low yields, and difficulties in purification. One of the most com…
Article
Re-use of Protein A Resin: Fouling and Economics
Mar 01, 2015
By Anurag S. Rathore, Mili Pathak, Guijun Ma, Daniel G. Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography ha…
Article
Tackling Analytical Method Development for ADCs
…a few methods may be required to support early conjugation process development, for example, SEC for aggregation, HIC for DAR, and RPC for free drug. Once the conjugation process has been established…
Article
SEC in the Modern Downstream Purification Process
Mar 01, 2015
By R. Christopher Manzari, J. Kevin O'Donnell
BioPharm International
Volume 3, Issue 28
Since the introduction of commercial chromatography resins approximately six de…
Article
FDA Approves First Biosimilar
These manufacturing differences could result in problems such as aggregation and could have the potential to create immune responses.
Legal issues linger
Amgen has alleged that Sandoz purposel…
Article
Fermentation for the Future
Improving efficiency, value chain, quality, and protein complexity with advanced bioprocess development.
Jan 01, 2015
By Cynthia Challener, PhD
Microorganisms—bacteria and yeast—have b…
Article
Technologies and Practices Must Evolve to Meet Demand
Jan 01, 2015 By Rita Peters
Through Dec. 3, 2014, nearly 30% of FDA’s new drug approvals for 2014 were for biologic-based therapies, up from 7% in 2013 (1). As the percentage of large-molecule …