Search results for " cell" in Articles / App Notes
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
In addition to high cell density mammalian expression, expanded utilization of other expression systems such as microbial and yeast will support these newer biotherapeutics (BioTx). The diversity of B…
Article
Leveraging Computational Models of Glycosylation for Biopharma QA
Large-scale mammalian cell culture is used for the manufacture of all therapeutic glycoproteins (TGPs), with Chinese hamster ovary (CHO) and murine myeloma (NS0 and Sp2/0) cell lines being the most-co…
Article
Impurity Testing of Biologic Drug Products
Some impurities (especially host cell proteins) may add an immune-stimulating or adjuvant behavior to the therapeutic, causing the patient to generate antibodies or cell-mediated immunity against the …
Article
Perfusion in the 21st Century
Batch processing, in which all raw materials are introduced into the bioreactor at the beginning of the cell-culture process, have largely been replaced with fed-batch systems, in which additional nut…
Article
Emerging Therapies Test Existing Bioanalytical Methods
Specialty assays used to understand the distribution, function, efficacy, and immunogenicity of gene- and cell-therapy drugs—including cell-based neutralizing assays, qPCR, flow cytometry and enzyme-l…
Article
An Integrated Approach to Ensure the Viral Safety of Biotherapeutics
By Mark Plavsic
Use of continuous cell lines in the manufacture of biological therapeutic products, such as vaccines, recombinant proteins, and monoclonal antibodies, is associated with the concom…
Article
Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
A typical bioprocess for a mammalian cell line may have 170 process factors. Using this approach, a 170-dimensional space would require 3.3 x 10119 cultures for full characterization, which is not fea…
Article
Analytical Considerations for Successful Upstream Process Development with Spivey and Lane
Topics covered include:
1:47 - Where the industry is headed with real-time analysis
3:17 - Scaling up
4:57 - Cell perfusion
7:16 - Criti…
Article
Cell-Culture Advances Test Bioreactor Performance Models
The continual development of higher-producing cell lines, the switch to chemically defined media, and other advances in cell-culture technology are, however, challenging developers of modeling and sim…
Article
Optimization of Protein Expression in Escherichia Coli
This population is typically comprised of a few cells expressing large amounts of the target of low quality, and the majority of the cells not expressing anything (4).
Codon usage differs among pr…