Search results for " cell"
Article
Essentials in Quality Risk Management
All unit operations, formulations, analytical methods, excipients, cell banks, working cell banks, equipment, reagents, chemistry, facilities, and materials are evaluated for the potential influence t…
Article
Considerations in depth filter scale-up
They are typically made of a mixture of cellulose, filter aids, and a binder. These components can be milled and mixed in different ratios to target a wide range of pore sizes. The complex nature of t…
Article
Reviewing the Importance of Biosimilarity and Interchangeability
Analytical studies for determining biological activity may involve in-vitro assays, cell-based assays, or other relevant biological assays depending on the mechanism of action of the drug, Stamm remar…
Article
In silico optimization of AAV full/empty particle separation with AIEX
The separation of adeno-associated virus (AAV) with high yield and purity is an increasingly important challenge. Mechanistic modeling is the tool of choice to find the optimal AIEX chromatogr…
Webcast
Unraveling the secrets of poloxamer 188 - A webinar mystery
When an inexplicable drop in cell culture performance is linked to a batch of media, researchers race to find the culprit.
In this on demand recording we will unravel the mystery as we journey thr…
Article
Exosome isolation by tangential flow filtration and size exclusion chromatography
We describe an evaluation of purification workflows from three different cell culture supernatants. The workflows we describe are suitable for scale-up and combine tangential flow filtration (TFF) for…
Article
Get Started: Purification of mAb Variants - eLearning
These molecules show wider variation in binding affinities and are often more challenging to separate from impurities, such as host cell proteins (HCP), host DNA, aggregates, and isoforms.
In this …
Article
Dear lentivirus: We have not forgotten about you and how to improve your polishing recovery
Lentiviruses are commonly used for both chimeric antigen receptor (CAR) T cell therapy and ex vivo gene therapy.
Vector purification is very challenging due to low stability of these envel…
Article
Balancing AAVs Versus LLVs
The prevailing understanding is that serotypes bind to different, or have differential affinities to an array of primary cell surface glycoprotein receptors and secondary receptors.
Pharmace…
Article
HTPD 2023 conference is coming up. What will be discussed and what happened last time?
Both the evolution of mature HTPD platforms for mAbs as well as the development of new HTPD approaches for nanoparticles like cells, viral vectors et al, will be discussed.
Learn more about HTPD …