Search results for " cell"
Article
Cleaning Protein A Resins with 1 M NaOH
The presence of cell culture nutrients in the harvest feed loaded on the capture column also exposes the protein A step to increased risk of microbial contamination. Historically, protein A resins hav…
Article
Going Viral
Manufacturing viral vector-based therapies such as vaccines and gene and cell therapies is complex, but a new manufacturing solution helps solve those challenges. The new KUBio BSL-2 solution for vira…
Article
3rd BioProcessing Asia Preview: Q&A with John Curling
…the opposite ends of the manufacturing scale: development of high-volume antibody processes and then cell based therapies.
As the monoclonal antibody industry moves toward larger patient groups …
Article
Process Chromatography: Continuous Optimization
Even so, there are many impurities present in the harvest cell-culture fluid obtained after removal of the cells or yeast and media. Host-cell proteins, host-cell DNA, other process-related contaminan…
Article
Hybrid Bioprocessing: A Q&A with Andrew Sinclair
Where the productivity of the cell line is equivalent in fed batch and perfusion, then the balance favors fed batch based on efficient media utilization and easier development. Perfusion has potential…
Article
Critical Quality Attributes Challenge Biologics Development
Determination of criticality of process and quality attributes improved significantly in the past decade with the advancement of knowledge in protein engineering, biology, and cell culture engineering…
Article
Speed and Flexibility Are Dual Goals for Biopharma Operations
The facilities, built in partnership with Cytiva, offer cGMP manufacturing based on mammalian cell culture technologies to meet customer demand for Phase II/III clinical trials. In addition, the singl…
Article
Impact of Manufacturing-Scale Freeze-Thaw Conditions on a mAb Solution
The measurement involved flushing of the flow cell with a 0.25 mL sample, followed by imaging analysis of a 0.65 mL sample. Reported values are the average of two samples, with an acceptance criterion…
Poster
Validation of the Production of Influenza Virus
As shear stress is a critical parameter in virus production from anchorage-dependent cells, the possibility to increase working volume to 4 L, without increasing shear forces, was investigated. The re…
Article
Validation of the production of influenza virus in ReadyToProcess WAVE™ 25 bioreactor system
As shear stress is a critical parameter in virus production from anchorage-dependent cells, the possibility to increase working volume to 4 L, without increasing shear forces, was investigated. The re…