Search results for " antibody"
Resource
Protein A Fiber Chromatography from Research to Scale-up and Manufacturing of Monoclonal Antibodies
The new Protein A fiber chromatography format allows for quick purification of many samples because of its short cycle times. This makes the pre-packed, small-scale Protein A fiber unit a versatile …
Article
Multidimensional Scale-Up of a Monoclonal Antibody Capture Step, Using ÄKTA™ pilot 600
Fast and efficient process development and scale-up contributes to a shortened time to market. In this work, a multidimensional scale-up (change of both column diameter and bed height) of a mAb capt…
Poster
Purification of monoclonal antibodies using modern chromatography media and membranes
This white paper is a guide to the development of MAb purification platforms and provides an overview of Cytiva’s offering of process chromatography media (resins) and membranes for MAb purification…
Article
Quality by design for biotechnology products—part 1
Switching the isotype of an antibody product to gain desired effector functions is one approach that has been practiced. In some cases, an IgG1 isotype is ideal for cell killing, whereas IgG2 is a fav…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
In the future, mAbs may represent a smaller percentage of the pipeline as portfolios concentrate more on development of antibody fragments, nanobodies, biosimilar protein therapeutics, conjugated prot…
Article
Host-Cell Protein Measurement and Control
In the case of an immunoassay, a polyclonal antibody (pAb) used in the test is generated by immunization with a preparation of a production cell minus the product-coding gene, fusion partners, or othe…
Article
A Closer Look at Affinity Ligands
Other examples include antibody/antigen, enzyme/substrate, and enzyme/inhibitor interactions. The degree of purification achieved by affinity purification can be quite high depending on the specificit…
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Fc receptors can be activating, or inhibitory, or without any effect in antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). Characterizing these highly complex…
Article
A Look at the Affinity Chromatography Landscape
They have also needed to develop resin chemistries more suitable for the growing range of protein-based biologics, such as fusion proteins and antibody fragments, and affinity ligands that can separat…
Article
Tackling Analytical Method Development for ADCs
The influence of conjugation on antibody binding must also be evaluated, and a wide range of stability studies must be conducted. Fortunately, both improvements in analytical technology and increased …