Search results for " E. coli"
Article
Optimization of Protein Expression in Escherichia Coli
Here, the authors discuss some parameters that can influence protein yields and quality during protein expression in E. coli.
Bacterial strain selection
The choice of a bacterial strain for prot…
Article
Scaling Up an E. coli Upstream Process for Plasmid DNA Production — from 2.5 to 160 L scale Fermenters
We used an E. coli DH5α strain which produced a pLP2 plasmid of 4180 bp in the study. Performance of peak E. coli growth measured as optical density at 600 nm (OD600), plasmid titer, and percentage of…
Article
Single-Use Workflow for Recovery of a Domain Antibody from E. coli Culture Feed in an Automated Manner
Here, a domain antibody (dAb) expressed in E. coli was recovered from fermentation broth. The dAb-containing sample was clarified (2.5-times concentration) and further washed with three wash volumes, …
Article
E. coli growth and Dab expression in single-use and stainless steel fermentors
This application note describes the performance of the Xcellerex™ single-use XDR-50 MO stirred-tank fermentor in an E. coli domain antibody (Dab) production process developed for a stainless steel …
Article
Antibody Production in Microbial Hosts
Of these, E. coli is the most popular host and two of the commercially available antibody-fragment products—Lucentis (ranibizumab injection) and Cimzia (cerolizumab pegol)—are expressed in E. coli (8)…
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Addressing the Challenges in Downstream Processing Today and Tomorrow
Cimzia and Lucentis are two recently approved Fabs produced in E. coli (29) and although no scFv has yet received regulatory approved, there are a large number of both types of Ab fragments in clinica…
Article
Cell-free Expression Systems Pose Cell Culture Alternative
“While the E. coli platform does not provide PTM at all, in fact, even in a wheat germ system or other eukaryotic systems, the PTM is still a concern for protein heterogeneity. Something might affect …
Article
Managing Residual Impurities During Downstream Processing
Atouf adds that in addition to the CHO and E. coli genomic DNA reference standards currently under development, additional reference standards may be added in the future based on demand. This chapter …
Article
Fermentation for the Future
Soluble expression
One of the drawbacks of many bacterial expression systems, particularly E. coli, is the formation of insoluble aggregates of the overexpressed recombinant protein, referred to a…
Article
Milestones and Moderate Progress in 2012 Drug Approvals
Voraxaze is a relatively large (83 kDa) homodimeric bacterial carboxypeptidase enzyme expressed in E. coli. It is approved for treatment of toxic plasma methotrexate concentrations in patients with de…